Product nameAnti-ATP1B3 antibody
See all ATP1B3 primary antibodies
DescriptionMouse polyclonal to ATP1B3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mammals
Full length human ATP1B3 protein
- ATP1B3 transfected 293T lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
ELISA pair antibody
Our Abpromise guarantee covers the use of ab67409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).|
FunctionThis is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Sequence similaritiesBelongs to the X(+)/potassium ATPases subunit beta family.
Cellular localizationMembrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- AT1B3_HUMAN antibody
- atp1b3 antibody
- ATPase Na+/K+ transporting beta 3 polypeptide antibody
All lanes : Anti-ATP1B3 antibody (ab67409) at 1/500 dilution
Lane 1 : ATP1B3 293T transfected lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Zhang SH et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). Read more (PubMed: 30792309) »
- Nishitsuji H et al. ATP1B3 Protein Modulates the Restriction of HIV-1 Production and Nuclear Factor ? Light Chain Enhancer of Activated B Cells (NF-?B) Activation by BST-2. J Biol Chem 291:4754-62 (2016). Read more (PubMed: 26694617) »