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Signal Transduction Metabolism Plasma Membrane ATPases
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RecombinantRabMAb

Recombinant Anti-ATP1B3 antibody [EPR8981] (ab137055)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
  • Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055)
  • Anti-ATP1B3 antibody [EPR8981] (ab137055)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8981] to ATP1B3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Recombinant Human ATP1B3 protein (ab157927)

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Overview

  • Product name

    Anti-ATP1B3 antibody [EPR8981]
    See all ATP1B3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8981] to ATP1B3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to amino acids near the C terminal of Human ATP1B3 (UniProt ID: P54709).

  • Positive control

    • A431 and HeLa cell lysates; Human cervical squamous carcinoma and Human liver tissues
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8981
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases

Associated products

  • Alternative Versions

    • Anti-ATP1B3 antibody [EPR8981] - BSA and Azide free (ab248791)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
    • A-431 membrane extract lysate (ab30134)
  • Recombinant Protein

    • Recombinant Human ATP1B3 protein (ab157927)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
    • Sequence similarities

      Belongs to the X(+)/potassium ATPases subunit beta family.
    • Cellular localization

      Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P54709 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 483 Human
      • Entrez Gene: 11933 Mouse
      • Entrez Gene: 25390 Rat
      • Omim: 601867 Human
      • SwissProt: P54709 Human
      • SwissProt: P97370 Mouse
      • SwissProt: Q63377 Rat
      • Unigene: 477789 Human
      • Unigene: 424 Mouse
      • Unigene: 5041 Rat
      see all
    • Alternative names

      • AT1B3_HUMAN antibody
      • atp1b3 antibody
      • ATPase Na+/K+ transporting beta 3 polypeptide antibody
      • ATPB 3 antibody
      • ATPB-3 antibody
      • ATPB3 antibody
      • CD 298 antibody
      • CD298 antibody
      • CD298 antigen antibody
      • FLJ29027 antibody
      • Na K ATPase beta 3 polypeptide antibody
      • Na+/K+ ATPase beta 3 subunit antibody
      • NKAB3S antibody
      • Sodium potassium ATPase subunit beta 3 (non catalytic) antibody
      • Sodium pump subunit beta 3 antibody
      • Sodium/potassium dependent ATPase beta 3 subunit antibody
      • Sodium/potassium dependent ATPase subunit beta 3 antibody
      • Sodium/potassium transporting ATPase beta 3 chain antibody
      • Sodium/potassium transporting ATPase subunit beta 3 antibody
      • Sodium/potassium-dependent ATPase subunit beta-3 antibody
      • Sodium/potassium-transporting ATPase subunit beta-3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)

      Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labelling ATP1B3 with ab137055 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ATP1B3 with ab137055 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      All lanes : Anti-ATP1B3 antibody [EPR8981] (ab137055) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 32 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Anti-ATP1B3 antibody [EPR8981] (ab137055)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab137055? Please let us know so that we can cite the reference in this datasheet.

    ab137055 has been referenced in 7 publications.

    • Zheng B  et al. ATP1B3 cooperates with BST-2 to promote hepatitis B virus restriction. J Med Virol 92:201-209 (2020). PubMed: 31556466
    • Ferri F  et al. Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements. Epigenetics Chromatin 12:46 (2019). PubMed: 31331374
    • Zhang SH  et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). PubMed: 30792309
    • Scudieri P  et al. Increased expression of ATP12A proton pump in cystic fibrosis airways. JCI Insight 3:N/A (2018). PubMed: 30333310
    • Li L  et al. Expression of the ß3 subunit of Na+/K+-ATPase is increased in gastric cancer and regulates gastric cancer cell progression and prognosis via the PI3/AKT pathway. Oncotarget 8:84285-84299 (2017). PubMed: 29137423
    • Rojas M  et al. Mineralocorticoids modulate the expression of the ß-3 subunit of the Na+, K+-ATPase in the renal collecting duct. Channels (Austin) 11:388-398 (2017). PubMed: 28636485
    • Marshall DJ  et al. Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins. Mol Ther N/A:N/A (2016). WB . PubMed: 27434591

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