Recombinant Anti-ATP1B3 antibody [EPR8981] (ab137055)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)](https://www.abcam.com/ps/products/137/ab137055/Images/ab137055-151557-anti-atp1b3-antibody-epr8981-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8981] to ATP1B3
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ATP1B3 antibody [EPR8981]
See all ATP1B3 primary antibodies -
Description
Rabbit monoclonal [EPR8981] to ATP1B3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to amino acids near the C terminal of Human ATP1B3 (UniProt ID: P54709).
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Positive control
- A431 and HeLa cell lysates; Human cervical squamous carcinoma and Human liver tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8981 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Application notes
Is unsuitable for Flow Cyt,ICC or IP.
Target
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Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known. -
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family. -
Cellular localization
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 483 Human
- Entrez Gene: 11933 Mouse
- Entrez Gene: 25390 Rat
- Omim: 601867 Human
- SwissProt: P54709 Human
- SwissProt: P97370 Mouse
- SwissProt: Q63377 Rat
- Unigene: 477789 Human
see all -
Alternative names
- AT1B3_HUMAN antibody
- atp1b3 antibody
- ATPase Na+/K+ transporting beta 3 polypeptide antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labelling ATP1B3 with ab137055 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)](https://www.abcam.com/ps/products/137/ab137055/Images/ab137055-151556-anti-atp1b3-antibody-epr8981-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ATP1B3 with ab137055 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055)](https://www.abcam.com/ps/products/137/ab137055/Images/ab137055-151558-anti-atp1b3-antibody-epr8981-western-blot.jpg)
Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055)All lanes : Anti-ATP1B3 antibody [EPR8981] (ab137055) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Anti-ATP1B3 antibody [EPR8981] (ab137055)](https://www.abcam.com/ps/products/137/ab137055/Images/ab137055-4-benefits-of-recombinant-antibodies.png)
Anti-ATP1B3 antibody [EPR8981] (ab137055)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab137055 has been referenced in 7 publications.
- Zheng B et al. ATP1B3 cooperates with BST-2 to promote hepatitis B virus restriction. J Med Virol 92:201-209 (2020). PubMed: 31556466
- Ferri F et al. Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements. Epigenetics Chromatin 12:46 (2019). PubMed: 31331374
- Zhang SH et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). PubMed: 30792309
- Scudieri P et al. Increased expression of ATP12A proton pump in cystic fibrosis airways. JCI Insight 3:N/A (2018). PubMed: 30333310
- Li L et al. Expression of the ß3 subunit of Na+/K+-ATPase is increased in gastric cancer and regulates gastric cancer cell progression and prognosis via the PI3/AKT pathway. Oncotarget 8:84285-84299 (2017). PubMed: 29137423
- Rojas M et al. Mineralocorticoids modulate the expression of the ß-3 subunit of the Na+, K+-ATPase in the renal collecting duct. Channels (Austin) 11:388-398 (2017). PubMed: 28636485
- Marshall DJ et al. Extracellular Antibody Drug Conjugates Exploiting the Proximity of Two Proteins. Mol Ther N/A:N/A (2016). WB . PubMed: 27434591