Recombinant
RabMAb

Recombinant Anti-ATP1B3 antibody [EPR8981] (ab137055)

Submit a review Submit a question References (5)

Overview

  • Product name

    Anti-ATP1B3 antibody [EPR8981]
    See all ATP1B3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8981] to ATP1B3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to amino acids near the C terminal of Human ATP1B3 (UniProt ID: P54709).

  • Positive control

    • A431 and HeLa cell lysates; Human cervical squamous carcinoma and Human liver tissues

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8981

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.

    • Sequence similarities

      Belongs to the X(+)/potassium ATPases subunit beta family.

    • Cellular localization

      Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AT1B3_HUMAN antibody
      • atp1b3 antibody
      • ATPase Na+/K+ transporting beta 3 polypeptide antibody
      • ATPB 3 antibody
      • ATPB-3 antibody
      • ATPB3 antibody
      • CD 298 antibody
      • CD298 antibody
      • CD298 antigen antibody
      • FLJ29027 antibody
      • Na K ATPase beta 3 polypeptide antibody
      • Na+/K+ ATPase beta 3 subunit antibody
      • NKAB3S antibody
      • Sodium potassium ATPase subunit beta 3 (non catalytic) antibody
      • Sodium pump subunit beta 3 antibody
      • Sodium/potassium dependent ATPase beta 3 subunit antibody
      • Sodium/potassium dependent ATPase subunit beta 3 antibody
      • Sodium/potassium transporting ATPase beta 3 chain antibody
      • Sodium/potassium transporting ATPase subunit beta 3 antibody
      • Sodium/potassium-dependent ATPase subunit beta-3 antibody
      • Sodium/potassium-transporting ATPase subunit beta-3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labelling ATP1B3 with ab137055 at 1/50 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ATP1B3 with ab137055 at 1/50 dilution.
    • Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055)
      All lanes : Anti-ATP1B3 antibody [EPR8981] (ab137055) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 32 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Zhang SH  et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). Read more (PubMed: 30792309) »
    • Scudieri P  et al. Increased expression of ATP12A proton pump in cystic fibrosis airways. JCI Insight 3:N/A (2018). Read more (PubMed: 30333310) »
    See all 5 Publications for this product

    Publishing research using ab137055? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab137055.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up