Key features and details
- Mouse monoclonal [7H10BD4F9] to ATP5A
- Suitable for: IHC-Fr, WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human, Zebrafish
- Isotype: IgG2b
Product nameAnti-ATP5A antibody [7H10BD4F9]
See all ATP5A primary antibodies
DescriptionMouse monoclonal [7H10BD4F9] to ATP5A
Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Zebrafish
Full length protein corresponding to Cow ATP5A.
- Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
Purification notesNear homogeneity as judged by SDS-PAGE. ab110273 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain typekappa
Our Abpromise guarantee covers the use of ab110273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23515448|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
Tissue specificityFetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Sequence similaritiesBelongs to the ATPase alpha/beta chains family.
modificationsThe N-terminus is blocked.
Cellular localizationMitochondrion inner membrane. Peripheral membrane protein.
- Information by UniProt
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
All lanes : Anti-ATP5A antibody [7H10BD4F9] (ab110273) at 1 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 60 kDa
HL-60 cells were stained with 1 µg/mL ab110273 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Mitochondrial localization of ATP5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110273 (2 µg/ml) followed by an Alexa Fluor® 594-conjugated-goat-anti-mouse IgG2b isotype specific secondary antibody (2 µg/ml).
ab110273 has been referenced in 56 publications.
- Ni S et al. Identification of ATP synthase a subunit as a new maternal factor capable of protecting zebrafish embryos from bacterial infection. FASEB J 33:12983-13001 (2019). PubMed: 31518507
- Perli E et al. Novel compound mutations in the mitochondrial translation elongation factor (TSFM) gene cause severe cardiomyopathy with myocardial fibro-adipose replacement. Sci Rep 9:5108 (2019). PubMed: 30911037
- Braganza AC et al. Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:N/A (2019). PubMed: 31120438
- Maio N et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). PubMed: 30765471
- Wang T et al. SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism. Mol Cell 75:823-834.e5 (2019). PubMed: 31302001