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    atp5a-antibody-ab222887.pdf

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Anti-ATP5A antibody (ab222887)

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Western blot - Anti-ATP5A antibody (ab222887)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody (ab222887)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody (ab222887)
  • Immunoprecipitation - Anti-ATP5A antibody (ab222887)

Key features and details

  • Rabbit polyclonal to ATP5A
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human ATP5A protein (Tagged) (ab235845)

View more associated products

Overview

  • Product name

    Anti-ATP5A antibody
    See all ATP5A primary antibodies
  • Description

    Rabbit polyclonal to ATP5A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human ATP5A aa 44-257.
    Database link: P25705

  • Positive control

    • WB: HeLa, K562, MCF7 and PC-3 whole cell lysates; Mouse heart lysate. IHC-P: Human liver and kidney tissues. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex V

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human ATP5A protein (Tagged) (ab235845)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
IHC-P
1/20 - 1/200.
IP
1/200 - 1/2000.
WB
1/1000 - 1/5000. Detects a band of approximately 60, 58 kDa (predicted molecular weight: 60 kDa).
Notes
IHC-P
1/20 - 1/200.
IP
1/200 - 1/2000.
WB
1/1000 - 1/5000. Detects a band of approximately 60, 58 kDa (predicted molecular weight: 60 kDa).

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
  • Tissue specificity

    Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
  • Sequence similarities

    Belongs to the ATPase alpha/beta chains family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Mitochondrion inner membrane. Peripheral membrane protein.
  • Target information above from: UniProt accession P25705 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 498 Human
    • Entrez Gene: 11946 Mouse
    • Omim: 164360 Human
    • SwissProt: P25705 Human
    • SwissProt: Q03265 Mouse
    • Unigene: 298280 Human
    • Unigene: 276137 Mouse
    • Unigene: 474505 Mouse
    • Alternative names

      • ATP synthase alpha chain antibody
      • ATP synthase alpha chain, mitochondrial antibody
      • ATP synthase subunit alpha antibody
      • ATP synthase subunit alpha mitochondrial antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
      • ATP sythase (F1 ATPase) alpha subunit antibody
      • ATP5A antibody
      • Atp5a1 antibody
      • ATP5AL2 antibody
      • ATPA_HUMAN antibody
      • ATPM antibody
      • Epididymis secretory sperm binding protein Li 123m antibody
      • hATP1 antibody
      • HEL-S-123m antibody
      • MC5DN4 antibody
      • mitochondrial antibody
      • Mitochondrial ATP synthetase antibody
      • Mitochondrial ATP synthetase oligomycin resistant antibody
      • Modifier of Min 2 antibody
      • Modifier of Min 2 mouse homolog antibody
      • Modifier of Min 2, mouse, homolog of antibody
      • MOM2 antibody
      • OMR antibody
      • ORM antibody
      • OTTHUMP00000163475 antibody
      see all

    Images

    • Western blot - Anti-ATP5A antibody (ab222887)
      Western blot - Anti-ATP5A antibody (ab222887)
      All lanes : Anti-ATP5A antibody (ab222887) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
      Lane 5 : Mouse heart lysate

      Secondary
      All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

      Predicted band size: 60 kDa
      Observed band size: 58,60 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody (ab222887)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody (ab222887)

      Paraffin-embedded human liver tissue stained for ATP5A using ab222887 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody (ab222887)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody (ab222887)

      Paraffin-embedded human kidney tissue stained for ATP5A using ab222887 at 1/100 dilution in immunohistochemical analysis.

    • Immunoprecipitation - Anti-ATP5A antibody (ab222887)
      Immunoprecipitation - Anti-ATP5A antibody (ab222887)

      ATP5A was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222887 at 1/200 dilution. 

      Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.

      Lane 2: ab222887 IP in HeLa whole cell lysate.

      Lane 3: HeLa whole cell lysate 20 µg (Input).

      For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab222887? Please let us know so that we can cite the reference in this datasheet.

    ab222887 has not yet been referenced specifically in any publications.

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