Product nameAnti-ATP5A antibody
See all ATP5A primary antibodies
DescriptionRabbit polyclonal to ATP5A
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human ATP5A aa 44-257.
Database link: P25705
- WB: HeLa, K562, MCF7 and PC-3 whole cell lysates; Mouse heart lysate. IHC-P: Human liver and kidney tissues. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|IP||1/200 - 1/2000.|
|WB||1/1000 - 1/5000. Detects a band of approximately 60, 58 kDa (predicted molecular weight: 60 kDa).|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
Tissue specificityFetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Sequence similaritiesBelongs to the ATPase alpha/beta chains family.
modificationsThe N-terminus is blocked.
Cellular localizationMitochondrion inner membrane. Peripheral membrane protein.
- Information by UniProt
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
All lanes : Anti-ATP5A antibody (ab222887) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 5 : Mouse heart lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 58,60 kDa why is the actual band size different from the predicted?
Paraffin-embedded human liver tissue stained for ATP5A using ab222887 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for ATP5A using ab222887 at 1/100 dilution in immunohistochemical analysis.
ATP5A was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222887 at 1/200 dilution.
Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.
Lane 2: ab222887 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
ab222887 has not yet been referenced specifically in any publications.