Name: Anti-ATP5A antibody [EPR13030(B)]
Brand: RabMAb
SKU: ab176569
Rating: 5 (4 reviews)

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Western blot - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13030(B)] to ATP5A
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

Product name

Anti-ATP5A antibody [EPR13030(B)]
See all ATP5A primary antibodies
Description

Rabbit monoclonal [EPR13030(B)] to ATP5A
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human ATP5A aa 200-300 (Cysteine residue). The exact sequence is proprietary. Isoform 1
Database link: P25705
Positive control
- HepG2, HeLa, fetal liver and fetal lung lysates; Human liver and fetal heart tissues; HeLa and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13030(B)
Isotype

IgG
Research areas
- Neuroscience
- Processes

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human ATP5A protein (Tagged) (ab235845)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF	(1)	1/100 - 1/250.
IHC-P		1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The use of an HRP/AP polymerized secondary antibody is recommended.
WB	(3)	1/1000 - 1/10000. Predicted molecular weight: 60 kDa.

Notes
Flow Cyt (Intra) 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100. The use of an HRP/AP polymerized secondary antibody is recommended.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
Tissue specificity

Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Sequence similarities

Belongs to the ATPase alpha/beta chains family.
Post-translational
modifications

The N-terminus is blocked.
Cellular localization

Mitochondrion inner membrane. Peripheral membrane protein.
Target information above from: UniProt accession P25705 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 498 Human
- Entrez Gene: 11946 Mouse
- Entrez Gene: 65262 Rat
- Omim: 164360 Human
- SwissProt: P25705 Human
- SwissProt: Q03265 Mouse
- SwissProt: P15999 Rat
- Unigene: 298280 Human
- Unigene: 276137 Mouse
- Unigene: 474505 Mouse
- Unigene: 40255 Rat
see all
Alternative names
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
- ATP synthase subunit alpha mitochondrial antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
- ATP sythase (F1 ATPase) alpha subunit antibody
- ATP5A antibody
- Atp5a1 antibody
- ATP5AL2 antibody
- ATPA_HUMAN antibody
- ATPM antibody
- Epididymis secretory sperm binding protein Li 123m antibody
- hATP1 antibody
- HEL-S-123m antibody
- MC5DN4 antibody
- mitochondrial antibody
- Mitochondrial ATP synthetase antibody
- Mitochondrial ATP synthetase oligomycin resistant antibody
- Modifier of Min 2 antibody
- Modifier of Min 2 mouse homolog antibody
- Modifier of Min 2, mouse, homolog of antibody
- MOM2 antibody
- OMR antibody
- ORM antibody
- OTTHUMP00000163475 antibody
see all

Western blot - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

All lanes : Anti-ATP5A antibody [EPR13030(B)] (ab176569) at 0.01 µg/ml

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 60 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Ab176569 (purified) staining ATP5A in HeLa (human cervix adenocarcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/500 dilution (4.2µg/ml). An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HeLa cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ATP5A with Purified ab176569 at 1:500 dilution (0.21 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ATP5A with purified ab176569 at 1/60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

All lanes : Anti-ATP5A antibody [EPR13030(B)] (ab176569) at 1/1000 dilution (unpurified)

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal lung lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 60 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Immunofluorescence analysis of MCF7 cells labeling ATP5A using ab176569 (unpurified) at a 1/100 dilution (green). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A using ab176569 (unpurified) at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling ATP5A using ab176569 (unpurified) at a 1/10 dilution (red) or a rabbit IgG negative control (green).
Anti-ATP5A antibody [EPR13030(B)] (ab176569)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab176569? Please let us know so that we can cite the reference in this datasheet.

ab176569 has been referenced in 18 publications.

Li Y et al. Sorafenib induces mitochondrial dysfunction and exhibits synergistic effect with cysteine depletion by promoting HCC cells ferroptosis. Biochem Biophys Res Commun 534:877-884 (2021). PubMed: 33162029
Chu Y et al. Glutathione peroxidase-1 overexpression reduces oxidative stress, and improves pathology and proteome remodeling in the kidneys of old mice. Aging Cell 19:e13154 (2020). PubMed: 32400101
Su C & Wang F Clinical and molecular findings in a family expressing a novel heterozygous variant of the G elongation factor mitochondrial 1 gene. Exp Ther Med 20:173 (2020). PubMed: 33093908
Zhang J et al. Molecular Profiling Reveals a Common Metabolic Signature of Tissue Fibrosis. Cell Rep Med 1:100056 (2020). PubMed: 33205063
Freyre CAC et al. MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes. Mol Cell 76:811-825.e14 (2019). PubMed: 31628041

View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Candida albicans Cell lysate - other (Cytosolic and mitochondrial fractions)

Gel Running Conditions

Reduced Denaturing (4-12% precast gel)

Loading amount

15 µg

Treatment

Induced with 10 mM proline for 1 h

Specification

Cytosolic and mitochondrial fractions

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Fitz Gerald S. Silao

Verified customer

Submitted Apr 27 2021

Application

Western blot

Sample

Mouse Tissue lysate - whole (kidney cortex)

Gel Running Conditions

Non-reduced Non-Denaturing (Native) (8-12% Bis-Tris)

Loading amount

50 µg

Specification

kidney cortex

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Nastaran Daneshgar

Verified customer

Submitted Jan 26 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (A549)

Permeabilization

Yes - 0.1% Triton X-100

Specification

A549

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Fixative

Methanol

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 06 2017

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse cells lines (3T3 fibroblasts, C2C12 myoblast)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

10 µg

Specification

Mouse cells lines (3T3 fibroblasts, C2C12 myoblast

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 21 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (18)

Customer reviews and Q&As

Western blot abreview for Anti-ATP5A antibody [EPR13030(B)]

Western blot abreview for Anti-ATP5A antibody [EPR13030(B)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-ATP5A antibody [EPR13030(B)]

Western blot abreview for Anti-ATP5A antibody [EPR13030(B)]

Post-translational
modifications