Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse cells lines (3T3 fibroblasts, C2C12 myoblast)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Mouse cells lines (3T3 fibroblasts, C2C12 myoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
1 - 3T3 cell lysate
2 - C2C12 cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Mar 21 2017