Application
Western blot
Sample
Candida albicans Cell lysate - other (Cytosolic and mitochondrial fractions)
Gel Running Conditions
Reduced Denaturing (4-12% precast gel)
Loading amount
15 µg
Treatment
Induced with 10 mM proline for 1 h
Specification
Cytosolic and mitochondrial fractions
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-rabbit poly-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal WestDura
Negative control
Distinct band is absent in the cytosolic fraction
Exposure
4 second(s)
Bands
Specific: approx. 59 kDa
Positive control
Bands were only observed in the mitochondrial fractions
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Fitz Gerald S. Silao
Verified customer
Submitted Apr 27 2021