Product nameAnti-ATP5A antibody [EPR13030(B)] (HRP)
See all ATP5A primary antibodies
DescriptionRabbit monoclonal [EPR13030(B)] to ATP5A (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: HepG2 and HeLa whole cell lysate (ab150035). Human adult liver tissue lysate. IHC-P: normal human liver tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Anti-ATP5A antibody [EPR13030(B)] (ab176569)
- Anti-ATP5A antibody [EPR13030(B)] (Alexa Fluor® 647) (ab196198)
- Anti-ATP5A antibody [EPR13030(B)] (Alexa Fluor® 488) (ab196467)
- Anti-ATP5A antibody [EPR13030(B)] (Alexa Fluor® 594) (ab216385)
- Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (ab231692)
Our Abpromise guarantee covers the use of ab195891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
Tissue specificityFetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
Sequence similaritiesBelongs to the ATPase alpha/beta chains family.
modificationsThe N-terminus is blocked.
Cellular localizationMitochondrion inner membrane. Peripheral membrane protein.
- Information by UniProt
- ATP synthase alpha chain antibody
- ATP synthase alpha chain, mitochondrial antibody
- ATP synthase subunit alpha antibody
All lanes : Anti-ATP5A antibody [EPR13030(B)] (HRP) (ab195891) at 1/5000 dilution
Lane 1 : HepG2 Whole Cell Lysate
Lane 2 : ATCC HeLa Whole Cell Lysate
Lane 3 : Human Adult Liver Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% milk blocking buffer before being incubated with ab195891 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of ATP5A staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195891 at 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre