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RecombinantRabMAb

Recombinant Anti-ATP5A antibody [EPR13031] (ab178421)

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Western blot - Anti-ATP5A antibody [EPR13031] (ab178421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13031] (ab178421)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13031] (ab178421)
  • Anti-ATP5A antibody [EPR13031] (ab178421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13031] to ATP5A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-ATP5A antibody [EPR13031]
    See all ATP5A primary antibodies
  • Description

    Rabbit monoclonal [EPR13031] to ATP5A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP5A aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P25705

  • Positive control

    • HeLa cells and cell lysates; HepG2 and Human fetal liver lysates; Human fetal heart tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13031
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex V

Associated products

  • Alternative Versions

    • Anti-ATP5A antibody [EPR13031] - BSA and Azide free (ab250045)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human ATP5A protein (Tagged) (ab235845)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
    • Tissue specificity

      Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
    • Sequence similarities

      Belongs to the ATPase alpha/beta chains family.
    • Post-translational
      modifications

      The N-terminus is blocked.
    • Cellular localization

      Mitochondrion inner membrane. Peripheral membrane protein.
    • Target information above from: UniProt accession P25705 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 498 Human
      • Omim: 164360 Human
      • SwissProt: P25705 Human
      • Unigene: 298280 Human
      • Alternative names

        • ATP synthase alpha chain antibody
        • ATP synthase alpha chain, mitochondrial antibody
        • ATP synthase subunit alpha antibody
        • ATP synthase subunit alpha mitochondrial antibody
        • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
        • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
        • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
        • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
        • ATP sythase (F1 ATPase) alpha subunit antibody
        • ATP5A antibody
        • Atp5a1 antibody
        • ATP5AL2 antibody
        • ATPA_HUMAN antibody
        • ATPM antibody
        • Epididymis secretory sperm binding protein Li 123m antibody
        • hATP1 antibody
        • HEL-S-123m antibody
        • MC5DN4 antibody
        • mitochondrial antibody
        • Mitochondrial ATP synthetase antibody
        • Mitochondrial ATP synthetase oligomycin resistant antibody
        • Modifier of Min 2 antibody
        • Modifier of Min 2 mouse homolog antibody
        • Modifier of Min 2, mouse, homolog of antibody
        • MOM2 antibody
        • OMR antibody
        • ORM antibody
        • OTTHUMP00000163475 antibody
        see all

      Images

      • Western blot - Anti-ATP5A antibody [EPR13031] (ab178421)
        Western blot - Anti-ATP5A antibody [EPR13031] (ab178421)
        All lanes : Anti-ATP5A antibody [EPR13031] (ab178421) at 1/1000 dilution

        Lane 1 : HepG2 cell lysates
        Lane 2 : HeLa cell lysates
        Lane 3 : Human fetal liver lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 60 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13031] (ab178421)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5A antibody [EPR13031] (ab178421)

        Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A with ab178421 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13031] (ab178421)
        Immunocytochemistry/ Immunofluorescence - Anti-ATP5A antibody [EPR13031] (ab178421)

        Immunofluorescent analysis of Hela cells labeling ATP5A with ab178421 at 1/100 dilution (red). DAPI nuclear staining (blue).

      • Anti-ATP5A antibody [EPR13031] (ab178421)
        Anti-ATP5A antibody [EPR13031] (ab178421)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab178421? Please let us know so that we can cite the reference in this datasheet.

      ab178421 has not yet been referenced specifically in any publications.

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