Key features and details
- Goat polyclonal to ATP5C1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-ATP5C1 antibody
See all ATP5C1 primary antibodies
DescriptionGoat polyclonal to ATP5C1
Specificityab190875 is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1). The immunizing peptide represents the N terminus of the mature protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Cow, Dog, Human, Cynomolgus monkey, Orangutan
- Rat skeletal muscle lysate.
This product was previously labelled as ATPG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab190875 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab190875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 33 kDa).
1 hour primary incubation is recommended for this product.
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Tissue specificityIsoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta.
Sequence similaritiesBelongs to the ATPase gamma chain family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- ATP synthase gamma chain, mitochondrial antibody
- ATP synthase subunit gamma antibody
- ATP synthase subunit gamma, mitochondrial antibody
Anti-ATP5C1 antibody (ab190875) at 0.1 µg/ml + Rat skeletal muscle lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab190875 has not yet been referenced specifically in any publications.