Product nameAnti-ATP5C1 antibody
See all ATP5C1 primary antibodies
DescriptionRabbit polyclonal to ATP5C1
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human ATP5C1 (internal sequence).
- HeLa and Jurkat cell extracts.
This product was previously labelled as ATPG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab74807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 33 kDa.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Tissue specificityIsoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta.
Sequence similaritiesBelongs to the ATPase gamma chain family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- ATP synthase gamma chain, mitochondrial antibody
- ATP synthase subunit gamma antibody
- ATP synthase subunit gamma, mitochondrial antibody
All lanes : Anti-ATP5C1 antibody (ab74807) at 1/500 dilution
Lane 1 : HeLa cell extracts
Lane 2 : Jurkat cell extracts
Lane 3 : HeLa cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 26 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
ab74807 has not yet been referenced specifically in any publications.