Key features and details
- Goat polyclonal to ATP5C1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-ATP5C1 antibody - C-terminal
See all ATP5C1 primary antibodies
DescriptionGoat polyclonal to ATP5C1 - C-terminal
Specificityab190310 is expected to recognize both reported isoforms (NP_005165.1; NP_001001973.1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog
- Human Heart and in Rat Heart lysates.
This product was previously labelled as ATPG
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab190310 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab190310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Tissue specificityIsoform Heart is expressed specifically in the heart and skeletal muscle, which require rapid energy supply. Isoform Liver is expressed in the brain, liver and kidney. Isoform Heart and Isoform Liver are expressed in the skin, intestine, stomach and aorta.
Sequence similaritiesBelongs to the ATPase gamma chain family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- ATP synthase gamma chain, mitochondrial antibody
- ATP synthase subunit gamma antibody
- ATP synthase subunit gamma, mitochondrial antibody
Immunohistchemical analysis of paraffin embedded Human heart tissue, staining ATP5C1 with ab190310 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Anti-ATP5C1 antibody - C-terminal (ab190310) at 0.01 µg/ml + Rat Heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 33 kDa
ab190310 has been referenced in 1 publication.
- Collins CM et al. ATP synthase F1 subunits recruited to centromeres by CENP-A are required for male meiosis. Nat Commun 9:2702 (2018). PubMed: 30006572