Anti-ATP5D antibody (ab97491)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-ATP5D antibody
See all ATP5D primary antibodies -
Description
Rabbit polyclonal to ATP5D -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 1-158 of Human ATP5D (NP_001678).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab97491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 17 kDa. | |
| IHC-P | 1/250. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
Sequence similarities
Belongs to the ATPase epsilon chain family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 338081 Cow
- Entrez Gene: 513 Human
- Entrez Gene: 66043 Mouse
- Entrez Gene: 245965 Rat
- Omim: 603150 Human
- SwissProt: P05630 Cow
- SwissProt: P30049 Human
- SwissProt: Q9D3D9 Mouse
see all -
Alternative names
- ATP synthase subunit delta, mitochondrial antibody
- ATP synthase subunit delta, mitochondrial antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (ab97491)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human breast cancer tissue, stained with ab97491 at 1/250.
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Western blot - Anti-ATP5D antibody (ab97491)All lanes : Anti-ATP5D antibody (ab97491) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 17 kDa
12% SDS PAGE -
Western blot - Anti-ATP5D antibody (ab97491)Anti-ATP5D antibody (ab97491) at 1/1000 dilution + Mouse brain at 50 µg
Predicted band size: 17 kDa
Protocols
References
This product has been referenced in:
- Senapati P et al. Hyperinsulinemia promotes aberrant histone acetylation in triple-negative breast cancer. Epigenetics Chromatin 12:44 (2019). Read more (PubMed: 31315653) »
- Liu X et al. Regulation of mitochondrial biogenesis in erythropoiesis by mTORC1-mediated protein translation. Nat Cell Biol 19:626-638 (2017). WB . Read more (PubMed: 28504707) »
