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Anti-ATP5D antibody (ab97491)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (ab97491)
  • Western blot - Anti-ATP5D antibody (ab97491)
  • Western blot - Anti-ATP5D antibody (ab97491)

Key features and details

  • Rabbit polyclonal to ATP5D
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human ATP5D protein (ab109956)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ATP5D antibody
    See all ATP5D primary antibodies
  • Description

    Rabbit polyclonal to ATP5D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-158 of Human ATP5D (NP_001678).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A431 whole cell lysate (ab7909)
    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human ATP5D protein (ab109956)

Applications

Our Abpromise guarantee covers the use of ab97491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.
IHC-P 1/250.

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Sequence similarities

    Belongs to the ATPase epsilon chain family.
  • Cellular localization

    Mitochondrion. Mitochondrion inner membrane.
  • Target information above from: UniProt accession P30049 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 338081 Cow
    • Entrez Gene: 513 Human
    • Entrez Gene: 66043 Mouse
    • Entrez Gene: 245965 Rat
    • Omim: 603150 Human
    • SwissProt: P05630 Cow
    • SwissProt: P30049 Human
    • SwissProt: Q9D3D9 Mouse
    • SwissProt: P35434 Rat
    • Unigene: 418668 Human
    • Unigene: 278560 Mouse
    • Unigene: 3879 Rat
    see all
  • Alternative names

    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase subunit delta, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit antibody
    • ATP5D antibody
    • ATPD_HUMAN antibody
    • F ATPase delta subunit antibody
    • F-ATPase delta subunit antibody
    • Mitochondrial ATP synthase complex delta subunit precusor antibody
    • Mitochondrial ATP synthase delta subunit antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (ab97491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5D antibody (ab97491)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human breast cancer tissue, stained with ab97491 at 1/250.

  • Western blot - Anti-ATP5D antibody (ab97491)
    Western blot - Anti-ATP5D antibody (ab97491)
    All lanes : Anti-ATP5D antibody (ab97491) at 1/500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 17 kDa



    12% SDS PAGE
  • Western blot - Anti-ATP5D antibody (ab97491)
    Western blot - Anti-ATP5D antibody (ab97491)
    Anti-ATP5D antibody (ab97491) at 1/1000 dilution + Mouse brain at 50 µg

    Predicted band size: 17 kDa

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab97491? Please let us know so that we can cite the reference in this datasheet.

    ab97491 has been referenced in 4 publications.

    • Senapati P  et al. Hyperinsulinemia promotes aberrant histone acetylation in triple-negative breast cancer. Epigenetics Chromatin 12:44 (2019). PubMed: 31315653
    • Tan JL  et al. New High-Throughput Screening Identifies Compounds That Reduce Viability Specifically in Liver Cancer Cells That Express High Levels of SALL4 by Inhibiting Oxidative Phosphorylation. Gastroenterology 157:1615-1629.e17 (2019). PubMed: 31446059
    • Liu X  et al. Regulation of mitochondrial biogenesis in erythropoiesis by mTORC1-mediated protein translation. Nat Cell Biol 19:626-638 (2017). WB . PubMed: 28504707
    • Engelen-Lee J  et al. Proteomic profiling of the spinal cord in ALS: decreased ATP5D levels suggest synaptic dysfunction in ALS pathogenesis. Amyotroph Lateral Scler Frontotemporal Degener 18:210-220 (2017). PubMed: 27899032

    Customer reviews and Q&As

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