Overview

  • Product name

  • Description

    Rabbit polyclonal to ATP5F1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human ATP5F1 aa 25-75. The exact sequence is proprietary.
    Sequence:

    PGVLQA TRTFHTGQPH LVPVPPLPEY GGKVRYGLIP EEFFQFLYPK TGVTG


    Database link: P24539

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH: 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab217054 was affinity purified using an epitope specific to ATP5F1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 29 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities

    Belongs to the eukaryotic ATPase B chain family.
  • Cellular localization

    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT5F1_HUMAN antibody
    • ATP synthase B chain mitochondrial antibody
    • ATP synthase subunit b antibody
    • ATP synthase subunit b mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibody
    • ATP5F1 antibody
    • ATPase subunit b antibody
    • Cell proliferation inducing protein 47 antibody
    • H+ ATP synthase subunit b antibody
    • MGC24431 antibody
    • mitochondrial antibody
    • PIG47 antibody
    see all

Images

  • All lanes : Anti-ATP5F1 antibody (ab217054) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : TCMK1 cell lysate
    Lane 5 : NIH 3T3 cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 29 kDa


    Exposure time: 10 seconds


    Cells prepared using NETN lysis buffer.

  • Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling ATP5F1 using ab217054 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated ATP5F1, ab217054 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab217054 has not yet been referenced specifically in any publications.

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