Key features and details
- Rabbit polyclonal to ATP5F1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ATP5F1 antibody
See all ATP5F1 primary antibodies
DescriptionRabbit polyclonal to ATP5F1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab217054 was affinity purified using an epitope specific to ATP5F1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab217054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 29 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Sequence similaritiesBelongs to the eukaryotic ATPase B chain family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- AT5F1_HUMAN antibody
- ATP synthase B chain mitochondrial antibody
- ATP synthase subunit b antibody
All lanes : Anti-ATP5F1 antibody (ab217054) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK1 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10 seconds
Cells prepared using NETN lysis buffer.
Immunoprecipitation analysis of 293T cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling ATP5F1 using ab217054 at 6 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated ATP5F1, ab217054 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab217054 has not yet been referenced specifically in any publications.