Key features and details
- Rabbit polyclonal to ATP5F1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ATP5F1 antibody
See all ATP5F1 primary antibodies
DescriptionRabbit polyclonal to ATP5F1
Specificityab217062 was affinity purified using an epitope specific to ATP5F1 immobilized on solid support.
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab217062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 29 kDa.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Sequence similaritiesBelongs to the eukaryotic ATPase B chain family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- AT5F1_HUMAN antibody
- ATP synthase B chain mitochondrial antibody
- ATP synthase subunit b antibody
Detection of ATP5F1 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217062 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217062 was used at 1µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes : Anti-ATP5F1 antibody (ab217062) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : mouse TCMK1 whole cell lysate
Lane 5 : mouse NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10 seconds
ab217062 has not yet been referenced specifically in any publications.