Anti-ATP5H antibody [10G5AB9] (ab173006)
![Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196454-anti-atp5h-antibody-10g5ab9-western-blot.jpg "Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)")
Key features and details
- Mouse monoclonal [10G5AB9] to ATP5H
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-ATP5H antibody [10G5AB9]
See all ATP5H primary antibodies -
Description
Mouse monoclonal [10G5AB9] to ATP5H -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Human, Rat, Mouse and Bovine Heart Homogenates; HeLa, HepG2, H4IIE, MEF, NIH-3T3 and COS7 cell lysates; HDFn cells; HeLa cells.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
10G5AB9 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
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| ICC/IF |
Human
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| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 18 kDa.
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 18 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. -
Sequence similarities
Belongs to the ATPase d subunit family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 282710 Cow
- Entrez Gene: 10476 Human
- Entrez Gene: 71679 Mouse
- Entrez Gene: 641434 Rat
- SwissProt: P13620 Cow
- SwissProt: O75947 Human
- SwissProt: Q9DCX2 Mouse
- SwissProt: P31399 Rat
see all -
Alternative names
- ATP synthase D chain mitochondrial antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
see all
Images
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Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.200000002980232 µg/ml
Lane 1 : Human Heart Homogenate at 20 µg
Lane 2 : Rat Heart Homogenate at 20 µg
Lane 3 : Mouse Heart Homogenate at 20 µg
Lane 4 : Bovine Heart Homogenate at 15 µg
Lane 5 : Hela cell lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 18 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196461-anti-atp5h-antibody-10g5ab9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)Immunocytochemistry with HDFn cells stained with ab173006 (red) and DAPI (blue).
Fixation: Paraformaldehyde fixed (4%, 20 min).
Used heated antigen retrieval for optimal signal.
Permeabilization: Triton X-100 permeabilized (0.1%, 10min).
Blocking: 10% Goat Serum/PBS (1 hour).
Primary: ab173006 5 µg/ml (10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C).
Secondary: Alexa Fluor® 594 goat anti-mouse H+L used at a 1/1000 dilution in 1% Goat Serum/PBS for 1hr at RT.
Washing: 3X 1% Goat Serum/PBS, 10 mins/wash.
DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins.
The target protein locates to the mitochondria (red).
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![Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196462-anti-atp5h-antibody-10g5ab9-flow-cytometry.jpg)
Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)Flow Cytometry with HeLa cells stained with ab173006 (blue) and isotype-specific negative control (red).
Fixation: Paraformaldehyde fixed (4%, 15 min).
Permeabilization: Methanol permeabilized (90%, @ -20°C for >30 min).
Blocking: 1% BSA /PBS (20 min).
Primary: ab173006 0.5 µg/ml in 1% BSA/PBS, 1hr at room temperature.
Secondary: Alexa Fluor® 488 goat anti-mouse IgG1 used at a 1/1000 dilution in 1% BSA/PBS for 30 min at RT.
Washing: 2x 1% BSA/PBS.
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![Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196457-anti-atp5h-antibody-10g5ab9-western-blot.jpg)
Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.5 µg/ml
Lane 1 : HepG2 cell lysate at 20 µg
Lane 2 : H4IIE cell lysate at 20 µg
Lane 3 : MEF cell lysate at 20 µg
Lane 4 : NIH-3T3 cell lysate at 20 µg
Lane 5 : COS7 cell lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 18 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab173006 has been referenced in 1 publication.
- Zhao Y et al. Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes. BMC Genomics 20:569 (2019). PubMed: 31291894