For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    atp5h-antibody-10g5ab9-ab173006.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Organelles Mitochondria
Share by email

Anti-ATP5H antibody [10G5AB9] (ab173006)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)
  • Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)
  • Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)

Key features and details

  • Mouse monoclonal [10G5AB9] to ATP5H
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human, African green monkey
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human ATP5H protein (ab153084)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-ATP5H antibody [10G5AB9]
    See all ATP5H primary antibodies
  • Description

    Mouse monoclonal [10G5AB9] to ATP5H
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Human, Rat, Mouse and Bovine Heart Homogenates; HeLa, HepG2, H4IIE, MEF, NIH-3T3 and COS7 cell lysates; HDFn cells; HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    10G5AB9
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex V

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-ATP5H antibody [10G5AB9] (ab198403)
    • Alexa Fluor® 488 Anti-ATP5H antibody [10G5AB9] (ab198594)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Mouse heart normal tissue lysate - total protein (ab30291)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human ATP5H protein (ab153084)
  • Related Products

    • Recombinant Human ATP5H protein (ab153084)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab173006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities

    Belongs to the ATPase d subunit family.
  • Cellular localization

    Mitochondrion. Mitochondrion inner membrane.
  • Target information above from: UniProt accession O75947 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282710 Cow
    • Entrez Gene: 10476 Human
    • Entrez Gene: 71679 Mouse
    • Entrez Gene: 641434 Rat
    • SwissProt: P13620 Cow
    • SwissProt: O75947 Human
    • SwissProt: Q9DCX2 Mouse
    • SwissProt: P31399 Rat
    • Unigene: 514465 Human
    • Unigene: 371641 Mouse
    • Unigene: 80 Rat
    see all
  • Alternative names

    • ATP synthase D chain mitochondrial antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
    • ATP synthase subunit d antibody
    • ATP synthase subunit d, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
    • ATP5H antibody
    • ATP5H_HUMAN antibody
    • ATP5JD antibody
    • ATPase subunit d antibody
    • ATPQ antibody
    • mitochondrial antibody
    • My032 protein antibody
    see all

Images

  • Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.200000002980232 µg/ml

    Lane 1 : Human Heart Homogenate at 20 µg
    Lane 2 : Rat Heart Homogenate at 20 µg
    Lane 3 : Mouse Heart Homogenate at 20 µg
    Lane 4 : Bovine Heart Homogenate at 15 µg
    Lane 5 : Hela cell lysate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)

    Immunocytochemistry with HDFn cells stained with ab173006 (red) and DAPI (blue).

     

    Fixation: Paraformaldehyde fixed (4%, 20 min).

    Used heated antigen retrieval for optimal signal.

    Permeabilization: Triton X-100 permeabilized (0.1%, 10min).

    Blocking: 10% Goat Serum/PBS (1 hour).

    Primary: ab173006 5 µg/ml (10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C).

    Secondary: Alexa Fluor® 594 goat anti-mouse H+L used at a 1/1000 dilution in 1% Goat Serum/PBS for 1hr at RT.

    Washing: 3X 1% Goat Serum/PBS, 10 mins/wash.

    DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins.

    The target protein locates to the mitochondria (red).

  • Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)

    Flow Cytometry with HeLa cells stained with ab173006 (blue) and isotype-specific negative control (red).

     

    Fixation: Paraformaldehyde fixed (4%, 15 min).

    Permeabilization: Methanol permeabilized (90%, @ -20°C for >30 min).

    Blocking: 1% BSA /PBS (20 min).

    Primary: ab173006 0.5 µg/ml in 1% BSA/PBS, 1hr at room temperature.

    Secondary: Alexa Fluor® 488 goat anti-mouse IgG1 used at a 1/1000 dilution in 1% BSA/PBS for 30 min at RT.

    Washing: 2x 1% BSA/PBS.

  • Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.5 µg/ml

    Lane 1 : HepG2 cell lysate at 20 µg
    Lane 2 : H4IIE cell lysate at 20 µg
    Lane 3 : MEF cell lysate at 20 µg
    Lane 4 : NIH-3T3 cell lysate at 20 µg
    Lane 5 : COS7 cell lysate at 10 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 18 kDa

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab173006? Please let us know so that we can cite the reference in this datasheet.

ab173006 has been referenced in 1 publication.

  • Zhao Y  et al. Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes. BMC Genomics 20:569 (2019). PubMed: 31291894

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ATP5H antibody [10G5AB9]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Retina)
Antigen retrieval step
Heat mediated
Specification
Retina
Blocking step
BSA+serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 03 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2021 Abcam plc. All rights reserved.