Anti-ATP5H antibody [10G5AB9] (ab173006)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-ATP5H antibody [10G5AB9]
See all ATP5H primary antibodies -
DescriptionMouse monoclonal [10G5AB9] to ATP5H
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Human, African green monkey
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Immunogen
Full length native protein (purified) corresponding to Cow ATP5H. Purified bovine OXPHOS complexes.
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Positive control
- Human, Rat, Mouse and Bovine Heart Homogenates; HeLa, HepG2, H4IIE, MEF, NIH-3T3 and COS7 cell lysates; HDFn cells; HeLa cells.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
ClonalityMonoclonal
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Clone number10G5AB9
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab173006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 µg/ml. Predicted molecular weight: 18 kDa. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| Flow Cyt | Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
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Sequence similaritiesBelongs to the ATPase d subunit family.
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Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 282710 Cow
- Entrez Gene: 10476 Human
- Entrez Gene: 71679 Mouse
- Entrez Gene: 641434 Rat
- SwissProt: P13620 Cow
- SwissProt: O75947 Human
- SwissProt: Q9DCX2 Mouse
- SwissProt: P31399 Rat
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Alternative names
- ATP synthase D chain mitochondrial antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
see all
Images
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![Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196454-ab173006a.jpg)
Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.200000002980232 µg/ml
Lane 1 : Human Heart Homogenate at 20 µg
Lane 2 : Rat Heart Homogenate at 20 µg
Lane 3 : Mouse Heart Homogenate at 20 µg
Lane 4 : Bovine Heart Homogenate at 15 µg
Lane 5 : Hela cell lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 18 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196461-ab173006c.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)Immunocytochemistry with HDFn cells stained with ab173006 (red) and DAPI (blue).
Fixation: Paraformaldehyde fixed (4%, 20 min).
Used heated antigen retrieval for optimal signal.
Permeabilization: Triton X-100 permeabilized (0.1%, 10min).
Blocking: 10% Goat Serum/PBS (1 hour).
Primary: ab173006 5 µg/ml (10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C).
Secondary: Alexa Fluor® 594 goat anti-mouse H+L used at a 1/1000 dilution in 1% Goat Serum/PBS for 1hr at RT.
Washing: 3X 1% Goat Serum/PBS, 10 mins/wash.
DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins.
The target protein locates to the mitochondria (red).
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![Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196462-ab173006d.jpg)
Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)Flow Cytometry with HeLa cells stained with ab173006 (blue) and isotype-specific negative control (red).
Fixation: Paraformaldehyde fixed (4%, 15 min).
Permeabilization: Methanol permeabilized (90%, @ -20°C for >30 min).
Blocking: 1% BSA /PBS (20 min).
Primary: ab173006 0.5 µg/ml in 1% BSA/PBS, 1hr at room temperature.
Secondary: Alexa Fluor® 488 goat anti-mouse IgG1 used at a 1/1000 dilution in 1% BSA/PBS for 30 min at RT.
Washing: 2x 1% BSA/PBS.
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![Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)](https://www.abcam.com/ps/products/173/ab173006/Images/ab173006-196457-ab173006b.jpg)
Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.5 µg/ml
Lane 1 : HepG2 cell lysate at 20 µg
Lane 2 : H4IIE cell lysate at 20 µg
Lane 3 : MEF cell lysate at 20 µg
Lane 4 : NIH-3T3 cell lysate at 20 µg
Lane 5 : COS7 cell lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 18 kDa
Protocols
Datasheets and documents
References
ab173006 has not yet been referenced specifically in any publications.
