Overview

  • Product name
    Anti-ATP5H antibody [10G5AB9]
    See all ATP5H primary antibodies
  • Description
    Mouse monoclonal [10G5AB9] to ATP5H
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, African green monkey
  • Immunogen

    Full length native protein (purified) corresponding to Cow ATP5H. Purified bovine OXPHOS complexes.

  • Positive control
    • Human, Rat, Mouse and Bovine Heart Homogenates; HeLa, HepG2, H4IIE, MEF, NIH-3T3 and COS7 cell lysates; HDFn cells; HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab173006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities
    Belongs to the ATPase d subunit family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ATP synthase D chain mitochondrial antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
    • ATP synthase subunit d antibody
    • ATP synthase subunit d, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
    • ATP5H antibody
    • ATP5H_HUMAN antibody
    • ATP5JD antibody
    • ATPase subunit d antibody
    • ATPQ antibody
    • mitochondrial antibody
    • My032 protein antibody
    see all

Images

  • Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.200000002980232 µg/ml

    Lane 1 : Human Heart Homogenate at 20 µg
    Lane 2 : Rat Heart Homogenate at 20 µg
    Lane 3 : Mouse Heart Homogenate at 20 µg
    Lane 4 : Bovine Heart Homogenate at 15 µg
    Lane 5 : Hela cell lysate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP5H antibody [10G5AB9] (ab173006)

    Immunocytochemistry with HDFn cells stained with ab173006 (red) and DAPI (blue).

     

    Fixation: Paraformaldehyde fixed (4%, 20 min).

    Used heated antigen retrieval for optimal signal.

    Permeabilization: Triton X-100 permeabilized (0.1%, 10min).

    Blocking: 10% Goat Serum/PBS (1 hour).

    Primary: ab173006 5 µg/ml (10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C).

    Secondary: Alexa Fluor® 594 goat anti-mouse H+L used at a 1/1000 dilution in 1% Goat Serum/PBS for 1hr at RT.

    Washing: 3X 1% Goat Serum/PBS, 10 mins/wash.

    DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins.

    The target protein locates to the mitochondria (red).

  • Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Flow Cytometry - Anti-ATP5H antibody [10G5AB9] (ab173006)

    Flow Cytometry with HeLa cells stained with ab173006 (blue) and isotype-specific negative control (red).

     

    Fixation: Paraformaldehyde fixed (4%, 15 min).

    Permeabilization: Methanol permeabilized (90%, @ -20°C for >30 min).

    Blocking: 1% BSA /PBS (20 min).

    Primary: ab173006 0.5 µg/ml in 1% BSA/PBS, 1hr at room temperature.

    Secondary: Alexa Fluor® 488 goat anti-mouse IgG1 used at a 1/1000 dilution in 1% BSA/PBS for 30 min at RT.

    Washing: 2x 1% BSA/PBS.

  • Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    Western blot - Anti-ATP5H antibody [10G5AB9] (ab173006)
    All lanes : Anti-ATP5H antibody [10G5AB9] (ab173006) at 0.5 µg/ml

    Lane 1 : HepG2 cell lysate at 20 µg
    Lane 2 : H4IIE cell lysate at 20 µg
    Lane 3 : MEF cell lysate at 20 µg
    Lane 4 : NIH-3T3 cell lysate at 20 µg
    Lane 5 : COS7 cell lysate at 10 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 18 kDa

References

ab173006 has not yet been referenced specifically in any publications.

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