• Product name
    Anti-ATP5H antibody [7F9BG1]
    See all ATP5H primary antibodies
  • Description
    Mouse monoclonal [7F9BG1] to ATP5H
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, African green monkey
  • Immunogen

    Other Immunogen Type corresponding to Cow ATP5H.

  • Positive control
    • Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
  • General notes

    This antibody clone is manufactured by Abcam.

    For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommneded with this antibody.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.



Our Abpromise guarantee covers the use of ab110275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.



  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities
    Belongs to the ATPase d subunit family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP synthase D chain mitochondrial antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
    • ATP synthase subunit d antibody
    • ATP synthase subunit d, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
    • ATP5H antibody
    • ATP5H_HUMAN antibody
    • ATP5JD antibody
    • ATPase subunit d antibody
    • ATPQ antibody
    • mitochondrial antibody
    • My032 protein antibody
    see all


  • All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1 µg/ml

    Lane 1 : HDFn (human) cell lysates at 20 µg
    Lane 2 : COS7 (monkey) cell lysates at 20 µg
    Lane 3 : H4IIE (rat) cell lysates at 20 µg
    Lane 4 : MEF (mouse) cell lysates at 20 µg
    Lane 5 : bovine heart mitochondria lysates at 5 µg

    Predicted band size: 18 kDa

  • All lanes : Anti-ATP5H antibody [7F9BG1] (ab110275) at 1/1 dilution

    Lane 1 : Human heart mitochondria at 10 µg
    Lane 2 : Bovine heart mitochondria at 4 µg
    Lane 3 : Rat heart mitochondria at 10 µg
    Lane 4 : Mouse heart mitochondria at 10 µg
    Lane 5 : HepG2 mitochondria at 20 µg

    Predicted band size: 18 kDa

  • Mitochondrial localization of ATP5H. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab110275 (5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG(H+L) secondary antibody (2 µg/ml).
  • HeLa cells were stained with 1 µg/mL ab110275 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.


This product has been referenced in:
  • Pecina P  et al. Role of the mitochondrial ATP synthase central stalk subunits ? and d in the activity and assembly of the mammalian enzyme. Biochim Biophys Acta 1859:374-381 (2018). Read more (PubMed: 29499186) »
  • Boominathan A  et al. Stable nuclear expression of ATP8 and ATP6 genes rescues a mtDNA Complex V null mutant. Nucleic Acids Res N/A:N/A (2016). Read more (PubMed: 27596602) »

See all 11 Publications for this product

Customer reviews and Q&As

For this antibody we have used a protocol with PFA and then a permeabilisation step with TritonX:

1. Fix the cells by incubating them in 4% (v/v) paraformaldehyde in PBS for 20 minutes at room


2. Rinse ...

Read More


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