Key features and details
- Rabbit polyclonal to ATP5H
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ATP5H antibody
See all ATP5H primary antibodies
DescriptionRabbit polyclonal to ATP5H
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse kidney, liver and brain lysates; HeLa, HepG2 and PC-3 whole cell lysates. IHC-P: Human brain and small intestine tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
Our Abpromise guarantee covers the use of ab230101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 16,18 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Sequence similaritiesBelongs to the ATPase d subunit family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- ATP synthase D chain mitochondrial antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
- ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
All lanes : Anti-ATP5H antibody (ab230101) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse brain lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 7 : U-251 MG (human brain glioma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 16,18 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Paraffin-embedded human brain tissue stained for ATP5H using ab230101 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for ATP5H using ab230101 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230101 has not yet been referenced specifically in any publications.