Anti-ATP5I antibody (ab122241)
Key features and details
- Rabbit polyclonal to ATP5I
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP5I antibody -
Description
Rabbit polyclonal to ATP5I -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence:
RRIAAEEKKKQDELKRIARELAEDDSILK
, corresponding to amino acids 41-69 of Human ATP5I. -
Positive control
- Human stomach tissue; RT-4 and U-251 MG cell lysates; Human Liver and Tonsil lysates; Human cell line A-431
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General notes
ab122241 is mono-specific.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
ab122241 is mono-specific. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. -
Sequence similarities
Belongs to the ATPase e subunit family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 521 Human
- Omim: 601519 Human
- SwissProt: P56385 Human
- Unigene: 85539 Human
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Alternative names
- ATP 5I antibody
- ATP 5K antibody
- ATP synthase e chain mitochondrial antibody
see all
Images
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All lanes : Anti-ATP5I antibody (ab122241) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
ab122241, at 1/200 dilution, staining ATP5I in paraffin-embedded Human stomach tissue by Immunohistochemistry.
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ab122241, at 4µg/ml, staining ATP5I in Human cell line A-431 by Immunofluorescence. Antibody staining is shown in green.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab122241 has been referenced in 4 publications.
- Morciano G et al. A naturally occurring mutation in ATP synthase subunit c is associated with increased damage following hypoxia/reoxygenation in STEMI patients. Cell Rep 35:108983 (2021). PubMed: 33852870
- Imasawa T et al. Proteomic Study of Low-Birth-Weight Nephropathy in Rats. Int J Mol Sci 22:N/A (2021). PubMed: 34638634
- Carraro M et al. The Unique Cysteine of F-ATP Synthase OSCP Subunit Participates in Modulation of the Permeability Transition Pore. Cell Rep 32:108095 (2020). PubMed: 32877677
- Mnatsakanyan N et al. A mitochondrial megachannel resides in monomeric F1FO ATP synthase. Nat Commun 10:5823 (2019). PubMed: 31862883