Key features and details
- Rabbit polyclonal to ATP5I
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ATP5I antibody
See all ATP5I primary antibodies
DescriptionRabbit polyclonal to ATP5I
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human stomach tissue; RT-4 and U-251 MG cell lysates; Human Liver and Tonsil lysates; Human cell line A-431
ab122241 is mono-specific.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesab122241 is mono-specific.
Our Abpromise guarantee covers the use of ab122241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Sequence similaritiesBelongs to the ATPase e subunit family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- ATP 5I antibody
- ATP 5K antibody
- ATP synthase e chain mitochondrial antibody
All lanes : Anti-ATP5I antibody (ab122241) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
ab122241, at 1/200 dilution, staining ATP5I in paraffin-embedded Human stomach tissue by Immunohistochemistry.
ab122241, at 4µg/ml, staining ATP5I in Human cell line A-431 by Immunofluorescence. Antibody staining is shown in green.
ab122241 has been referenced in 1 publication.
- Mnatsakanyan N et al. A mitochondrial megachannel resides in monomeric F1FO ATP synthase. Nat Commun 10:5823 (2019). PubMed: 31862883