Product nameAnti-ATP5I antibody
See all ATP5I primary antibodies
DescriptionRabbit polyclonal to ATP5I
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human ATP5I aa 19-69. The exact sequence is proprietary.
Database link: P56385
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysate. IP: HEK-293T cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
Our Abpromise guarantee covers the use of ab226393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 8 kDa.|
FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Sequence similaritiesBelongs to the ATPase e subunit family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
- ATP 5I antibody
- ATP 5K antibody
- ATP synthase e chain mitochondrial antibody
All lanes : Anti-ATP5I antibody (ab226393) at 0.2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 8 kDa
Exposure time: 10 seconds
ATP5I was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 1.0 mg for IP, 20% of IP loaded) with ab226393 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226393 at 1 µg/ml.
Lane 1: ab226393 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
ab226393 has not yet been referenced specifically in any publications.