Anti-ATP5J antibody (ab154578)
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Overview
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Product nameAnti-ATP5J antibody
See all ATP5J primary antibodies -
DescriptionRabbit polyclonal to ATP5J
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-108 of Human ATP5J (P18859).
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Positive control
- HeLa whole cell lysate and HeLa membrane lysate. Hela cells.
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General notesKeep as concentrated solution.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79% PBS, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 12 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
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Sequence similaritiesBelongs to the eukaryotic ATPase subunit F6 family.
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Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 522 Human
- Omim: 603152 Human
- SwissProt: P18859 Human
- Unigene: 246310 Human
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Alternative names
- ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
- ATP synthase-coupling factor 6, mitochondrial antibody
- ATP synthase-coupling factor 6, mitochondrial antibody
see all
Images
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Western blot - Anti-ATP5J antibody (ab154578)All lanes : Anti-ATP5J antibody (ab154578) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa membrane lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa
15% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP5J antibody (ab154578)Immunohistochemical analysis of paraffin-embedded J5 xenograft, labelling ATP5J using ab154578 at 1/500. -
Immunocytochemistry/ Immunofluorescence - Anti-ATP5J antibody (ab154578)Immunofluorescence analysis of methanol-fixed HeLa cells, labelling ATP5J using: (1) ab154578 at 1/500, (2) Co-stained with Hoechst 33342.
Protocols
References
ab154578 has not yet been referenced specifically in any publications.
