Anti-ATP5J2 antibody (ab200715)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ATP5J2 antibody -
Description
Rabbit polyclonal to ATP5J2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human ATP5J2 aa 32-77. The exact sequence is proprietary.
Sequence:MRDFSPSGIFGAFQRGYYRYYNKYINVKKGSISGITMVLACYVLFS
Database link: P56134 -
Positive control
- HEK293T, Raw 264.7 and H9C2 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab200715 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab200715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
Target
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Relevance
ATP5J2 is part of the complex F0 domain of the mitochondrial ATP synthase. This produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of two complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. ATP5J2 encodes the f subunit of the Fo complex. There are 2 isoforms produced by alternative splicing. -
Cellular localization
Mitochondrial; Mitochondrion inner membrane -
Database links
- Entrez Gene: 9551 Human
- Entrez Gene: 57423 Mouse
- Entrez Gene: 100173467 Orangutan
- Entrez Gene: 690441 Rat
- SwissProt: P56134 Human
- SwissProt: P56135 Mouse
- SwissProt: Q5R6T5 Orangutan
- SwissProt: D3ZAF6 Rat
see all -
Alternative names
- ATP synthase f chain, mitochondrial antibody
- ATP synthase subunit f, mitochondrial antibody
- ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2 antibody
see all
Images
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Western blot - Anti-ATP5J2 antibody (ab200715)All lanes : Anti-ATP5J2 antibody (ab200715) at 1/500 dilution
Lane 1 : HEK293T whole cell lysate
Lane 2 : Raw 264.7 whole cell lysate
Lane 3 : H9C2 whole cell lysate
Predicted band size: 11 kDa
Protocols
References
ab200715 has not yet been referenced specifically in any publications.
