Product nameAnti-ATP5L2 + ATP5L antibody [EPR15636]
See all ATP5L2 + ATP5L primary antibodies
DescriptionRabbit monoclonal [EPR15636] to ATP5L2 + ATP5L
Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human ATP5L2 + ATP5L aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q7Z4Y8
- HeLa, Human fetal liver and Human pancreas lysates; Human kidney tissue; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceMitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.
- ATP synthase subunit g 2, mitochondrial antibody
- ATP synthase subunit g, mitochondrial antibody
- ATP5K2 antibody
Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-ATP5L2 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.
All lanes : Anti-ATP5L2 + ATP5L antibody [EPR15636] (ab191417) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human pancreas tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 11 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP5L2 + ATP5L with ab191417 at 1/100 dilution (19.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab191417 at 1/100 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
ab191417 has not yet been referenced specifically in any publications.