Overview

  • Product name

  • Description

    Rabbit polyclonal to ATP5O
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ATP5O aa 38-64.
    Sequence:

    EGRYATALYSAASKQNKLEQVEKELLR


    Database link: P48047

  • Positive control

    • IHC-P:Human skeletal muscle tissue. WB: HEK-293 cell lysate. Flow: HEK-293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 23 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • Sequence similarities

    Belongs to the ATPase delta chain family.
  • Cellular localization

    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP synthase O subunit mitochondrial precursor antibody
    • ATP synthase subunit O antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody
    • ATP5O antibody
    • ATPO antibody
    • ATPO_HUMAN antibody
    • mitochondrial antibody
    • Mitochondrial ATP synthase, O subunit antibody
    • Oligomycin sensitivity conferral protein antibody
    • OSCP antibody
    see all

Images

  • Anti-ATP5O antibody (ab230325) at 1/1000 dilution + HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 35 µg

    Predicted band size: 23 kDa

  • Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for ATP50 using ab230325 (5 µg/ml) in immunohistochemical analysis.

  • Flow cytometric analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling ATP50 with ab230325 at 1/10 dilution (green) compared with a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • All lanes : Anti-ATP5O antibody (ab230325) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : ATP50-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 23 kDa

  • Paraffin-embedded human skeletal muscle stained for ATP5O (green) using ab230325 (0.05 mg/ml) in immunohistochemical analysis.

    FITC-conjugated goat anti-rabbit lgG was used as the secondary antibody. Counterstained with Propidium Iodide (red).

References

ab230325 has not yet been referenced specifically in any publications.

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