Overview

  • Product name
  • Description
    Rabbit polyclonal to ATP6AP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human ATP6AP1 aa 44-235.
    Sequence:

    QVPLVLW SSDRDLWAPA ADTHEGHITS DLQLSTYLDP ALELGPRNVL LFLQDKLSIE DFTAYGGVFG NKQDSAFSNL ENALDLAPSS LVLPAVDWYA VSTLTTYLQE KLGASPLHVD LATLRELKLN ASLPALLLIR LPYTASSGLM APREVLTGND EVIGQVLSTL KSEDVPYTAA LTAVRPSRVA RDVAV


    Database link: Q15904

  • Positive control
    • Fetal kidney lysate, human brain tissue

Properties

  • Form
    Lyophilised:200 ul distilled water
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/500.

Target

  • Relevance
    ATP6AP1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme, which is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase dependent organelle acidification is necessary for intracellular processes such as receptor-mediated endocytosis, protein sorting and zymogen activation.
  • Cellular localization
    Vacuole membrane. Single-pass membrane protein.
  • Database links
  • Alternative names
    • 16A antibody
    • Ac45 antibody
    • ATP6IP1 antibody
    • ATP6S1 antibody
    • ATPase H+ transporting lysosomal (vacuolar proton pump) subunit 1 antibody
    • ATPase H+ transporting lysosomal accessory protein 1 antibody
    • ATPase H+ transporting lysosomal interacting protein 1 antibody
    • CF2 antibody
    • H ATPase subunit antibody
    • MGC129781 antibody
    • ORF antibody
    • Protein XAP 3 antibody
    • Protein XAP3 antibody
    • V ATPase Ac45 subunit antibody
    • V ATPase S1 accessory protein antibody
    • V ATPase subunit S1 antibody
    • V type proton ATPase subunit S1 antibody
    • Vacuolar ATP synthase subunit S1 antibody
    • Vacuolar proton pump subunit S1 antibody
    • VATPS1 antibody
    • XAP 3 antibody
    • XAP3 antibody
    see all

Images

  • Immunohistochemical staining of formalin-fixed paraffin-embedded Human brain tissue labeling ATP6AP1 with ab176609 at 1/100 dilution showing membrane staining.

  • Anti-ATP6AP1 antibody (ab176609) at 1/500 dilution + Fetal kidney lysate

    Predicted band size: 52 kDa

References

This product has been referenced in:
  • Rujano MA  et al. Mutations in the X-linked ATP6AP2 cause a glycosylation disorder with autophagic defects. J Exp Med 214:3707-3729 (2017). Read more (PubMed: 29127204) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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