Anti-ATP6V0D2 antibody (ab194557)
Key features and details
- Mouse polyclonal to ATP6V0D2
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-ATP6V0D2 antibody
See all ATP6V0D2 primary antibodies -
Description
Mouse polyclonal to ATP6V0D2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human ATP6V0D2 aa 200 to the C-terminus. NP_689778
Database link: Q8N8Y2 -
Positive control
- Y79 cell lysate; recombinant immunogen
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/500 - 1/2500. Predicted molecular weight: 40 kDa.
|
Notes |
---|
WB
1/500 - 1/2500. Predicted molecular weight: 40 kDa. |
Target
-
Relevance
Vacuolar ATPase (V ATPase) is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). It is responsible for acidifying a variety of intracellular organelles in eukaryotic cells. ATP6V0D2 is a component of the integral membrane V0 complex of vacuolar ATPase and may have a role in coupling of proton transport and ATP hydrolysis. ATP6V0D2 may be involved in regulation of osteoclast fusion and bone formation. -
Cellular localization
Apical plasma membrane -
Database links
- Entrez Gene: 245972 Human
- Entrez Gene: 242341 Mouse
- Entrez Gene: 297932 Rat
- SwissProt: Q8N8Y2 Human
- SwissProt: Q80SY3 Mouse
- SwissProt: Q5FVL0 Rat
-
Alternative names
- ATP6D2 antibody
- ATPase H+ transporting lysosomal 38kDa V0 subunit D antibody
- ATPase H+ transporting lysosomal 38kDa V0 subunit D isoform 2 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab194557 has been referenced in 2 publications.
- Liu N et al. Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2a-mediated tumor progression. J Clin Invest N/A:N/A (2019). PubMed: 30431439
- Zhu X et al. V?9Vd2 T cells inhibit immature dendritic cell transdifferentiation into osteoclasts through downregulation of RANK, c-Fos and ATP6V0D2. Int J Mol Med 42:2071-2079 (2018). PubMed: 30066839