Anti-ATP6V1A antibody [8B1F3] (ab113745)

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Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745)

    Key features and details

    • Mouse monoclonal [8B1F3] to ATP6V1A
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-ATP6V1A antibody [8B1F3]
      See all ATP6V1A primary antibodies

    • Description

      Mouse monoclonal [8B1F3] to ATP6V1A

    • Host species

      Mouse

    • Tested Applications & Species

      Application Species
      WB
      Saccharomyces cerevisiae
      See all applications and species data

    • Immunogen

      Full length native protein (purified). This information is considered to be commercially sensitive.

    • General notes

      This antibody clone is manufactured by Abcam.

      Product was previously marketed under the MitoSciences sub-brand.

      If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab113745 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Saccharomyces cerevisiae
    Application Abreviews Notes
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
    Notes
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

    Target

    • Function

      Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

    • Tissue specificity

      Present in all tissues analyzed.

    • Sequence similarities

      Belongs to the ATPase alpha/beta chains family.
    • Information by UniProt

    • Alternative names

      • 70kDa subunit antibody
      • ATP6A1 antibody
      • ATP6V1A antibody
      • ATP6V1A1 antibody
      • ATPase H+ transporting lysosomal subunit A1 antibody
      • ATPase H+ transporting, lysosomal 70kDa V1 subunit A antibody
      • H(+) transporting two sector ATPase subunit A antibody
      • H+ transporting ATPase chain A vacuolar (VA68 type) antibody
      • HO68 antibody
      • OTTHUMP00000214746 antibody
      • V ATPase 69 kDa subunit 1 antibody
      • V ATPase A subunit 1 antibody
      • V ATPase subunit A 1 antibody
      • V-ATPase 69 kDa subunit antibody
      • V-ATPase subunit A antibody
      • V-type proton ATPase catalytic subunit A antibody
      • VA68 antibody
      • Vacuolar ATP synthase catalytic subunit A ubiquitous isoform antibody
      • Vacuolar ATPase isoform VA68 antibody
      • Vacuolar proton pump alpha subunit 1 antibody
      • Vacuolar proton pump subunit alpha antibody
      • VATA_HUMAN antibody
      • Vma1 antibody
      • VPP2 antibody
      see all

    Images

    • Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745)
      Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745)
      Anti-ATP6V1A antibody [8B1F3] (ab113745) at 1 µg/ml + Yeast Whole Cell at 15 µg

      Predicted band size: 69 kDa

    References (2)

    Publishing research using ab113745? Please let us know so that we can cite the reference in this datasheet.

    ab113745 has been referenced in 2 publications.

    • Chandel A & Bachhawat AK Redox regulation of the yeast voltage-gated Ca2+ channel homolog Cch1p by glutathionylation of specific cysteine residues. J Cell Sci 130:2317-2328 (2017). PubMed: 28576969
    • Chandel A  et al. Glutathione depletion activates the yeast vacuolar transient receptor potential channel, Yvc1p, by reversible glutathionylation of specific cysteines. Mol Biol Cell 27:3913-3925 (2016). IF . PubMed: 27708136

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