Anti-ATP6V1A antibody [8B1F3] (ab113745)
Key features and details
- Mouse monoclonal [8B1F3] to ATP6V1A
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG2a
Overview
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Product name
Anti-ATP6V1A antibody [8B1F3]
See all ATP6V1A primary antibodies -
Description
Mouse monoclonal [8B1F3] to ATP6V1A -
Host species
Mouse -
Tested Applications & Species
Application Species WB Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
8B1F3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Saccharomyces cerevisiae
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Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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Notes |
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WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
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Function
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
Tissue specificity
Present in all tissues analyzed. -
Sequence similarities
Belongs to the ATPase alpha/beta chains family. - Information by UniProt
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Alternative names
- 70kDa subunit antibody
- ATP6A1 antibody
- ATP6V1A antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab113745 has been referenced in 2 publications.
- Chandel A & Bachhawat AK Redox regulation of the yeast voltage-gated Ca2+ channel homolog Cch1p by glutathionylation of specific cysteine residues. J Cell Sci 130:2317-2328 (2017). PubMed: 28576969
- Chandel A et al. Glutathione depletion activates the yeast vacuolar transient receptor potential channel, Yvc1p, by reversible glutathionylation of specific cysteines. Mol Biol Cell 27:3913-3925 (2016). IF . PubMed: 27708136