Anti-ATP6V1A antibody [8B1F3] (ab113745)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-ATP6V1A antibody [8B1F3]
See all ATP6V1A primary antibodies -
DescriptionMouse monoclonal [8B1F3] to ATP6V1A
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Saccharomyces cerevisiae
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Immunogen
Full length native protein (purified)
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesNear homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
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ClonalityMonoclonal
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Clone number8B1F3
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab113745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). | |
| ICC/IF | Use a concentration of 20 µg/ml. heat induced antigen retrieval recommended |
Target
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FunctionCatalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
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Tissue specificityPresent in all tissues analyzed.
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Sequence similaritiesBelongs to the ATPase alpha/beta chains family.
- Information by UniProt
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Alternative names
- 70kDa subunit antibody
- ATP6A1 antibody
- ATP6V1A antibody
see all
Images
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![Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745)](https://www.abcam.com/ps/products/113/ab113745/Images/V-type-proton-ATPase-catalytic-subunit-A-Primary-antibodies-ab113745-1.jpg)
Western blot - Anti-ATP6V1A antibody [8B1F3] (ab113745)Anti-ATP6V1A antibody [8B1F3] (ab113745) at 1 µg/ml + Yeast Whole Cell at 15 µg
Predicted band size: 69 kDa
Datasheets and documents
References
This product has been referenced in:
- Chandel A et al. Glutathione depletion activates the yeast vacuolar transient receptor potential channel, Yvc1p, by reversible glutathionylation of specific cysteines. Mol Biol Cell 27:3913-3925 (2016). IF . Read more (PubMed: 27708136) »
