Anti-ATP6V1A antibody (ab137574)
Key features and details
- Rabbit polyclonal to ATP6V1A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ATP6V1A antibody
See all ATP6V1A primary antibodies -
Description
Rabbit polyclonal to ATP6V1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 60-344 of Human ATP6V1A (Uniprot ID P38606).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 68 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 68 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
Tissue specificity
Present in all tissues analyzed. -
Sequence similarities
Belongs to the ATPase alpha/beta chains family. - Information by UniProt
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Database links
- Entrez Gene: 282147 Cow
- Entrez Gene: 523 Human
- Entrez Gene: 11964 Mouse
- Entrez Gene: 445531 Pig
- Entrez Gene: 685232 Rat
- Omim: 607027 Human
- SwissProt: P31404 Cow
- SwissProt: P38606 Human
see all -
Alternative names
- 70kDa subunit antibody
- ATP6A1 antibody
- ATP6V1A antibody
see all
Images
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All lanes : Anti-ATP6V1A antibody (ab137574) at 1/10000 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : Hep G2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa
7.5% SDS PAGE -
Anti-ATP6V1A antibody (ab137574) at 1/5000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed A431 cells labelled with ab137574 at 1/500 dilution. Costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab137574 has been referenced in 14 publications.
- Liu N et al. ATP6V0d2 Suppresses Alveoli Macrophage Alternative Polarization and Allergic Asthma via Degradation of PU.1. Allergy Asthma Immunol Res 13:479-497 (2021). PubMed: 33733641
- Aoto K et al. ATP6V0A1 encoding the a1-subunit of the V0 domain of vacuolar H+-ATPases is essential for brain development in humans and mice. Nat Commun 12:2107 (2021). PubMed: 33833240
- Cheng A et al. ATM loss disrupts the autophagy-lysosomal pathway. Autophagy 17:1998-2010 (2021). PubMed: 32757690
- Wang L et al. The lysosomal membrane protein Sidt2 is a vital regulator of mitochondrial quality control in skeletal muscle. FASEB J 35:e21223 (2021). PubMed: 33715196
- Rao S et al. Salmonella and S. aureus Escape From the Clearance of Macrophages via Controlling TFEB. Front Microbiol 11:573844 (2020). PubMed: 33324360