Overview

  • Product name

  • Description

    Rabbit polyclonal to ATP6V1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 60-344 of Human ATP6V1A (Uniprot ID P38606).

  • Positive control

    • HeLa, HepG2 & mouse brain whole cell lysate, A431 cells, 293T, H1299, Molt-4, Raji

Properties

Applications

Our Abpromise guarantee covers the use of ab137574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/5000 - 1/20000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
  • Tissue specificity

    Present in all tissues analyzed.
  • Sequence similarities

    Belongs to the ATPase alpha/beta chains family.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70kDa subunit antibody
    • ATP6A1 antibody
    • ATP6V1A antibody
    • ATP6V1A1 antibody
    • ATPase H+ transporting lysosomal subunit A1 antibody
    • ATPase H+ transporting, lysosomal 70kDa V1 subunit A antibody
    • H(+) transporting two sector ATPase subunit A antibody
    • H+ transporting ATPase chain A vacuolar (VA68 type) antibody
    • HO68 antibody
    • OTTHUMP00000214746 antibody
    • V ATPase 69 kDa subunit 1 antibody
    • V ATPase A subunit 1 antibody
    • V ATPase subunit A 1 antibody
    • V-ATPase 69 kDa subunit antibody
    • V-ATPase subunit A antibody
    • V-type proton ATPase catalytic subunit A antibody
    • VA68 antibody
    • Vacuolar ATP synthase catalytic subunit A ubiquitous isoform antibody
    • Vacuolar ATPase isoform VA68 antibody
    • Vacuolar proton pump alpha subunit 1 antibody
    • Vacuolar proton pump subunit alpha antibody
    • VATA_HUMAN antibody
    • Vma1 antibody
    • VPP2 antibody
    see all

Images

  • All lanes : Anti-ATP6V1A antibody (ab137574) at 1/10000 dilution

    Lane 1 : Hela whole cell lysate
    Lane 2 : Hep G2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Anti-ATP6V1A antibody (ab137574) at 1/5000 dilution + Mouse brain whole cell lysate at 20 µg

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A431 cells labelled with ab137574 at 1/500 dilution. Costained with Hoechst 33342.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Immuno-precipitation step
Other - Prot A/G-Dynabeads
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Total protein in input
1000 µg

Abcam user community

Verified customer

Submitted Apr 08 2014

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