Recombinant
RabMAb

Recombinant Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839)

Overview

  • Product name

    Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401]
    See all ATP6V1B1 + ATP6V1B2 primary antibodies
  • Description

    Rabbit monoclonal [EPR16401] to ATP6V1B1 + ATP6V1B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP6V1B1 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P15313

  • Positive control

    • WB: JAR and RAW 264.7 cell lysates; Mouse brain, mouse kidney and rat kidney tissue lysates. IHC-P: Human, mouse and rat kidney tissues. ICC/IF: HEK293 and JAR cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16401
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab200839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.

Images

  • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized JAR (Human placenta choriocarcinoma cell line) cells labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on JAR cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200839 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on HEK293 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200839 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839) at 1/5000 dilution + JAR (Human placenta choriocarcinoma cell line) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-ATP6V1B1 + ATP6V1B2 antibody [EPR16401] (ab200839) at 1/1000 dilution

    Lane 1 : JAR (Human placenta choriocarcinoma cell line) cell lysate
    Lane 2 : JAR (Human placenta choriocarcinoma cell line) cell lysate with immunizing peptide
    Lane 3 : JAR (Human placenta choriocarcinoma cell line) cell lysate with ATP6V1B2 peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling ATP6V1B1 + ATP6V1B2 with ab200839 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human cardiac muscle tissue represents a negative control for ATP6V1B1 + ATP6V1B2. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

References

This product has been referenced in:

  • Song XB  et al. Autophagy blockade and lysosomal membrane permeabilization contribute to lead-induced nephrotoxicity in primary rat proximal tubular cells. Cell Death Dis 8:e2863 (2017). Read more (PubMed: 28594408) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab200839.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up