Recombinant
RabMAb

Recombinant Anti-ATP6V1B1 antibody [EPR16400] (ab192612)

Overview

  • Product name

    Anti-ATP6V1B1 antibody [EPR16400]
    See all ATP6V1B1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16400] to ATP6V1B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ATP6V1B1 aa 100-200. The exact sequence is proprietary.
    Database link: P15313

  • Positive control

    • JAR, Human fetal kidney, Mouse kidney and spleen, Rat kidney lysates; Human, Mouse and Rat kidney tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
  • Tissue specificity

    Expressed in the cochlea and endolymphatic sac.
  • Involvement in disease

    Defects in ATP6V1B1 are the cause of distal renal tubular acidosis with deafness (dRTA-D) [MIM:267300]. Inheritance is autosomal recessive. Patients with recessive dRTA are severely affected, presenting with either acute illness or growth failure at a young age, and bilateral sensorineural deafness. Other features include low serum K(+) due to renal potassium wasting, and elevated urinary calcium. If untreated, this acidosis may result in dissolution of bone, leading to osteomalacia and rickets. Renal deposition of calcium salts (nephrocalcinosis) and renal stone formation commonly occur.
  • Sequence similarities

    Belongs to the ATPase alpha/beta chains family.
  • Domain

    The PDZ-binding motif mediates interactions with SLC9A3R1 and SCL4A7.
  • Cellular localization

    Endomembrane system. Endomembrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atp6b1 antibody
    • ATP6V1B1 antibody
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta 56/58 kDa, isoform 1 antibody
    • ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 1 antibody
    • ATPase, H+ transporting, V1 subunit B, isoform 1 antibody
    • Endomembrane proton pump 58 kDa subunit antibody
    • H(+) transporting two sector ATPase 58kD subunit antibody
    • H+ ATPase beta 1 subunit antibody
    • kidney isoform antibody
    • Lysosomal 56/58kDa antibody
    • MGC32642 antibody
    • RTA1B antibody
    • V ATPase B1 subunit antibody
    • V type proton ATPase subunit B kidney isoform antibody
    • V type proton ATPase subunit B, kidney isoform antibody
    • V-ATPase subunit B 1 antibody
    • V-type proton ATPase subunit B antibody
    • Vacuolar proton pump 3 antibody
    • Vacuolar proton pump subunit 3 antibody
    • Vacuolar proton pump subunit B 1 antibody
    • VATB antibody
    • VATB1_HUMAN antibody
    • VMA2 antibody
    • Vpp 3 antibody
    • Vpp3 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP6V1B1 with ab192612 at 1/500 dilution  (1.4 μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-ATP6V1B1 antibody [EPR16400] (ab192612) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

  • Anti-ATP6V1B1 antibody [EPR16400] (ab192612) at 1/1000 dilution + JAR cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

  • Anti-ATP6V1B1 antibody [EPR16400] (ab192612) at 1/10000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 57 kDa

References

ab192612 has not yet been referenced specifically in any publications.

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