Product nameAnti-ATP6V1C1 antibody
See all ATP6V1C1 primary antibodies
DescriptionRabbit polyclonal to ATP6V1C1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- HepG2 cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab87163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionSubunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Sequence similaritiesBelongs to the V-ATPase C subunit family.
- Information by UniProt
- ATP6C antibody
- ATP6D antibody
- ATP6V1C1 antibody
Anti-ATP6V1C1 antibody (ab87163) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
This product has been referenced in:
- Farsi Z et al. Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity. Elife 7:N/A (2018). Read more (PubMed: 29652249) »
- Sarkar J et al. V-type ATPase proton pump expression during enamel formation. Matrix Biol 52-54:234-245 (0). Read more (PubMed: 26586472) »