Anti-ATP6V1E1 antibody (ab111733)
Key features and details
- Rabbit polyclonal to ATP6V1E1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ATP6V1E1 antibody
See all ATP6V1E1 primary antibodies -
Description
Rabbit polyclonal to ATP6V1E1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
-
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 160 of Human ATP6V1E1 (UniProt ID: P36543).
-
Positive control
- A549 and Mouse brain whole cell lysates; HeLa cells
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab111733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 26 kDa. | |
| ICC/IF | 1/100 - 1/500. |
Target
-
Relevance
Function: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Tissue specificity: Ubiquitous. Similarity: Belongs to the V-ATPase E subunit family. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 287017 Cow
- Entrez Gene: 529 Human
- Entrez Gene: 8992 Human
- Entrez Gene: 11973 Mouse
- Entrez Gene: 11974 Mouse
- Entrez Gene: 297566 Rat
- Entrez Gene: 94170 Rat
- Omim: 108746 Human
see all -
Alternative names
- ATP6E antibody
- ATP6E2 antibody
- ATP6V1E antibody
see all
Images
-
Western blot - Anti-ATP6V1E1 antibody (ab111733)Anti-ATP6V1E1 antibody (ab111733) at 1/3000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Western blot - Anti-ATP6V1E1 antibody (ab111733)Anti-ATP6V1E1 antibody (ab111733) at 1/1000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1E1 antibody (ab111733)Immunofluorescence analysis of ATP6V1E1 in methanol-fixed HeLa cells, using ab111733 at a 1/200 dilution. Lower image shows the cells counter stained with Hoechst 33342.
Protocols
References (5)
ab111733 has been referenced in 5 publications.
- Ponsford AH et al. Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor. Autophagy N/A:1-19 (2020). PubMed: 32515674
- Farsi Z et al. Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity. Elife 7:N/A (2018). PubMed: 29652249
- Doyle T et al. The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nat Microbiol 3:1369-1376 (2018). PubMed: 30478388
- Cao X et al. V-ATPase promotes transforming growth factor-ß-induced epithelial-mesenchymal transition of rat proximal tubular epithelial cells. Am J Physiol Renal Physiol 302:F1121-32 (2012). WB, ICC/IF ; Rat . PubMed: 22129967
- Sarkar J et al. V-type ATPase proton pump expression during enamel formation. Matrix Biol 52-54:234-245 (0). PubMed: 26586472
