Anti-ATP6V1E1 antibody (ab111733)
- Datasheet
- References (4)
- Protocols
Overview
-
Product name
Anti-ATP6V1E1 antibody
See all ATP6V1E1 primary antibodies -
Description
Rabbit polyclonal to ATP6V1E1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
-
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 160 of Human ATP6V1E1 (UniProt ID: P36543).
-
Positive control
- A549 and Mouse brain whole cell lysates; HeLa cells
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab111733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 26 kDa. | |
| ICC/IF | 1/100 - 1/500. |
Target
-
Relevance
Function: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Tissue specificity: Ubiquitous. Similarity: Belongs to the V-ATPase E subunit family. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 287017 Cow
- Entrez Gene: 529 Human
- Entrez Gene: 8992 Human
- Entrez Gene: 11973 Mouse
- Entrez Gene: 11974 Mouse
- Entrez Gene: 297566 Rat
- Entrez Gene: 94170 Rat
- Omim: 108746 Human
see all -
Alternative names
- ATP6E antibody
- ATP6E2 antibody
- ATP6V1E antibody
see all
Images
-
Western blot - Anti-ATP6V1E1 antibody (ab111733)Anti-ATP6V1E1 antibody (ab111733) at 1/3000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Western blot - Anti-ATP6V1E1 antibody (ab111733)Anti-ATP6V1E1 antibody (ab111733) at 1/1000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1E1 antibody (ab111733)Immunofluorescence analysis of ATP6V1E1 in methanol-fixed HeLa cells, using ab111733 at a 1/200 dilution. Lower image shows the cells counter stained with Hoechst 33342.
References
This product has been referenced in:
- Farsi Z et al. Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity. Elife 7:N/A (2018). Read more (PubMed: 29652249) »
- Doyle T et al. The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Nat Microbiol 3:1369-1376 (2018). Read more (PubMed: 30478388) »
