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    atp6v1f-antibody-ab155927.pdf

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Signal Transduction Metabolism Plasma Membrane ATPases
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Anti-ATP6V1F antibody (ab155927)

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Western blot - Anti-ATP6V1F antibody (ab155927)

    Key features and details

    • Rabbit polyclonal to ATP6V1F
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human ATP6V1F protein (ab202196)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-ATP6V1F antibody
      See all ATP6V1F primary antibodies
    • Description

      Rabbit polyclonal to ATP6V1F
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow
    • Immunogen

      Recombinant fragment, corresponding to a region within amino acids 1-119 of Human ATP6V1F (Uniprot ID: Q16864).

    • Positive control

      • U87-MG whole cell lysate
    • General notes

      Keep as a concentrated solution.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • ATPases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human ATP6V1F protein (ab202196)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab155927 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 13 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 13 kDa.

    Target

    • Function

      Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
    • Sequence similarities

      Belongs to the V-ATPase F subunit family.
    • Target information above from: UniProt accession Q16864 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 282405 Cow
      • Entrez Gene: 9296 Human
      • Entrez Gene: 66144 Mouse
      • Entrez Gene: 116664 Rat
      • Omim: 607160 Human
      • SwissProt: Q28029 Cow
      • SwissProt: Q16864 Human
      • SwissProt: Q9D1K2 Mouse
      • SwissProt: P50408 Rat
      • Unigene: 78089 Human
      see all
    • Alternative names

      • Adenosinetriphosphatase 14k chain antibody
      • ATP6S14 antibody
      • ATP6V1F antibody
      • ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F antibody
      • ATPase, vacuolar, 14 kD antibody
      • H(+)-transporting two-sector ATPase, 14kD subunit antibody
      • MGC117321 antibody
      • MGC126037 antibody
      • MGC126038 antibody
      • V-ATPase 14 kDa subunit antibody
      • V-ATPase subunit F antibody
      • V-type proton ATPase subunit F antibody
      • Vacuolar ATP synthase subunit F antibody
      • Vacuolar proton pump subunit F antibody
      • VATF antibody
      • VATF_HUMAN antibody
      • Vma7 antibody
      see all

    Images

    • Western blot - Anti-ATP6V1F antibody (ab155927)
      Western blot - Anti-ATP6V1F antibody (ab155927)
      Anti-ATP6V1F antibody (ab155927) at 1/500 dilution + U87-MG whole cell lysate at 30 µg

      Predicted band size: 13 kDa



      15% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab155927? Please let us know so that we can cite the reference in this datasheet.

    ab155927 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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