Overview

  • Product name

    Anti-ATP6V1F antibody [EPR15053]
    See all ATP6V1F primary antibodies
  • Description

    Rabbit monoclonal [EPR15053] to ATP6V1F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP6V1F aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16864

  • Positive control

    • Human fetal kidney and Mouse kidney lysates; A549, HeLa and MCF7 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15053
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 13 kDa.
IP 1/100.

Target

Images

  • All lanes : Anti-ATP6V1F antibody [EPR15053] (ab190789) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Anti-ATP6V1F antibody [EPR15053] (ab190789) at 1/1000 dilution + Mouse kidney lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Immunoprecipitation analysis of Human fetal kidney lysate labeling ATP6V1F using ab190789 at 1/100 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution was used as secondary antibody.

References

ab190789 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190789.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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