Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15053] to ATP6V1F
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Product nameAnti-ATP6V1F antibody [EPR15053]
See all ATP6V1F primary antibodies
DescriptionRabbit monoclonal [EPR15053] to ATP6V1F
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP6V1F aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q16864
- Human fetal kidney and Mouse kidney lysates; A549, HeLa and MCF7 cell lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab190789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 13 kDa.|
FunctionSubunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Sequence similaritiesBelongs to the V-ATPase F subunit family.
- Information by UniProt
- Adenosinetriphosphatase 14k chain antibody
- ATP6S14 antibody
- ATP6V1F antibody
All lanes : Anti-ATP6V1F antibody [EPR15053] (ab190789) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Anti-ATP6V1F antibody [EPR15053] (ab190789) at 1/1000 dilution + Mouse kidney lysate at 10 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Immunoprecipitation analysis of Human fetal kidney lysate labeling ATP6V1F using ab190789 at 1/100 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution was used as secondary antibody.
ab190789 has not yet been referenced specifically in any publications.