Anti-ATP6V1G3 antibody (ab122012)
Key features and details
- Rabbit polyclonal to ATP6V1G3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP6V1G3 antibody -
Description
Rabbit polyclonal to ATP6V1G3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
amino acids 36-115 (AKEEAMVEID QYRMQRDKEF RLKQSKIMGS QNNLSDEIEE QTLGKIQELN GHYNKYMESV MNQLLSMVCD MKPEIHVNYR) of Human ATP6V1G3 (Q96LB4).
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Positive control
- WB: RT-4, U-251 MG, Human liver and tonsil lysate. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/250 - 1/500.
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IHC-P |
1/5000 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/250 - 1/500. |
IHC-P
1/5000 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
Tissue specificity
Kidney. -
Sequence similarities
Belongs to the V-ATPase G subunit family. - Information by UniProt
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Database links
- Entrez Gene: 127124 Human
- SwissProt: Q96LB4 Human
- Unigene: 127743 Human
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Alternative names
- ATP6G3 antibody
- atp6v1g3 antibody
- ATPase, H+ transporting, lysosomal (vacuolar proton pump) subunit G3 antibody
see all
Images
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All lanes : Anti-ATP6V1G3 antibody (ab122012) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human plasma.
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human kidney tissue labeling ATP6V1G3 with ab122012 at 1/5000 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab122012 has been referenced in 1 publication.
- Heller EA et al. Locus-specific epigenetic remodeling controls addiction- and depression-related behaviors. Nat Neurosci 17:1720-7 (2014). PubMed: 25347353