Anti-ATP6V1H antibody (ab187706)
Key features and details
- Rabbit polyclonal to ATP6V1H
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ATP6V1H antibody
See all ATP6V1H primary antibodies -
Description
Rabbit polyclonal to ATP6V1H -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
-
Immunogen
Synthetic peptide within Human ATP6V1H aa 400-450. The exact sequence is proprietary.
Database link: NP_057025.2 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab187706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. Predicted molecular weight: 56 kDa. |
Target
-
Function
Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the V-ATPase H subunit family. - Information by UniProt
-
Database links
- Entrez Gene: 282657 Cow
- Entrez Gene: 486953 Dog
- Entrez Gene: 51606 Human
- Entrez Gene: 108664 Mouse
- Entrez Gene: 397472 Pig
- Entrez Gene: 297797 Rat
- Entrez Gene: 693323 Rhesus monkey
- Omim: 608861 Human
see all -
Alternative names
- Atp6v1h antibody
- ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H antibody
- CGI 11 antibody
see all
Images
-
Western blot - Anti-ATP6V1H antibody (ab187706)Lane 1: Detection of Human ATP6V1H by Immunoprecipitation from HeLa whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab187706 at 6 µg per reaction.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab187706 at 1 µg/ml. -
Western blot - Anti-ATP6V1H antibody (ab187706)All lanes : Anti-ATP6V1H antibody (ab187706) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse cell lysate
Lane 5 : Mouse NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 30 seconds
Datasheets and documents
References (2)
ab187706 has been referenced in 2 publications.
- Yambire KF et al. Impaired lysosomal acidification triggers iron deficiency and inflammation in vivo. Elife 8:N/A (2019). PubMed: 31793879
- Farsi Z et al. Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity. Elife 7:N/A (2018). PubMed: 29652249
