Product nameAnti-ATP6V1H antibody
See all ATP6V1H primary antibodies
DescriptionRabbit Polyclonal to ATP6V1H
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide within Human ATP6V1H aa 400-450. The exact sequence is proprietary.
Database link: NP_057025.2
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 cell lysates. HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 56 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSubunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the V-ATPase H subunit family.
- Information by UniProt
- Atp6v1h antibody
- ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H antibody
- CGI 11 antibody
Lane 1: Detection of Human ATP6V1H by Immunoprecipitation from HeLa whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab187706 at 6 µg per reaction.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab187706 at 1 µg/ml.
All lanes : Anti-ATP6V1H antibody (ab187706) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse cell lysate
Lane 5 : Mouse NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 30 seconds