Anti-ATP6V1H antibody (ab187706)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-ATP6V1H antibody
See all ATP6V1H primary antibodies -
Description
Rabbit Polyclonal to ATP6V1H -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human ATP6V1H aa 400-450. The exact sequence is proprietary.
Database link: NP_057025.2 -
Positive control
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 cell lysates. HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab187706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. Predicted molecular weight: 56 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the V-ATPase H subunit family. - Information by UniProt
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Database links
- Entrez Gene: 282657 Cow
- Entrez Gene: 486953 Dog
- Entrez Gene: 51606 Human
- Entrez Gene: 108664 Mouse
- Entrez Gene: 397472 Pig
- Entrez Gene: 297797 Rat
- Entrez Gene: 693323 Rhesus monkey
- Omim: 608861 Human
see all -
Alternative names
- Atp6v1h antibody
- ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H antibody
- CGI 11 antibody
see all
Images
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Western blot - Anti-ATP6V1H antibody (ab187706)Lane 1: Detection of Human ATP6V1H by Immunoprecipitation from HeLa whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab187706 at 6 µg per reaction.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab187706 at 1 µg/ml. -
Western blot - Anti-ATP6V1H antibody (ab187706)All lanes : Anti-ATP6V1H antibody (ab187706) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse cell lysate
Lane 5 : Mouse NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 30 seconds
Datasheets and documents
References
This product has been referenced in:
- Farsi Z et al. Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity. Elife 7:N/A (2018). Read more (PubMed: 29652249) »
