Overview

  • Product name

  • Description

    Rabbit Polyclonal to ATP6V1H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human ATP6V1H aa 400-450. The exact sequence is proprietary.
    Database link: NP_057025.2

  • Positive control

    • HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 cell lysates. HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 56 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the V-ATPase H subunit family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Atp6v1h antibody
    • ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H antibody
    • CGI 11 antibody
    • CGI11 antibody
    • EC 3.6.3.14 antibody
    • MSTP042 antibody
    • NBP1 antibody
    • Nef binding protein 1 antibody
    • Nef-binding protein 1 antibody
    • Protein VMA13 homolog antibody
    • SFD antibody
    • SFDalpha antibody
    • SFDbeta antibody
    • V ATPase subunit H antibody
    • V-ATPase 50/57 kDa subunits antibody
    • V-ATPase subunit H antibody
    • V-type proton ATPase subunit H antibody
    • Vacuolar ATP synthase subunit H antibody
    • Vacuolar proton pump subunit H antibody
    • Vacuolar proton pump subunit SFD antibody
    • VATH_HUMAN antibody
    • VMA13 antibody
    see all

Images

  • Lane 1: Detection of Human ATP6V1H by Immunoprecipitation from HeLa whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab187706 at 6 µg per reaction.
    Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
    Subsequent Western blot detection used ab187706 at 1 µg/ml.

  • All lanes : Anti-ATP6V1H antibody (ab187706) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Mouse cell lysate
    Lane 5 : Mouse NIH3T3 cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 30 seconds

References

This product has been referenced in:

See 1 Publication for this product

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