Anti-ATP7b antibody (ab133731)
Key features and details
- Rabbit polyclonal to ATP7b
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP7b antibody
See all ATP7b primary antibodies -
Description
Rabbit polyclonal to ATP7b -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide from the N-Terminus of Human ATP7b.
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Positive control
- Human lung (respiratory epithelium) tissue or HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 48% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. -
Tissue specificity
Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. -
Involvement in disease
Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
Contains 6 HMA domains. -
Post-translational
modificationsIsoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form. -
Cellular localization
Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels. - Information by UniProt
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Database links
- Entrez Gene: 540 Human
- Entrez Gene: 11979 Mouse
- Entrez Gene: 24218 Rat
- Omim: 606882 Human
- SwissProt: P35670 Human
- SwissProt: Q64446 Mouse
- SwissProt: Q64535 Rat
- Unigene: 492280 Human
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Alternative names
- ATP7B antibody
- ATP7B_HUMAN antibody
- ATPase, Cu(2+) transporting, beta polypeptide antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin embedded Human lung (respiratory epithelium) tissue labelling ATP7b with ab133731 at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunofluorescence analysis of HeLa cells, labelling ATP7b with ab133731 at a 1/100 dilution (left image), or immunizing peptide (right image).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133731 has been referenced in 1 publication.
- Tian H et al. Effects of Salvia miltiorrhiza extract on lung adenocarcinoma. Exp Ther Med 22:794 (2021). PubMed: 34093750