Recombinant
RabMAb

Recombinant Anti-ATP7b antibody [EPR6793] (ab131208)

Overview

  • Product name

    Anti-ATP7b antibody [EPR6793]
    See all ATP7b primary antibodies
  • Description

    Rabbit monoclonal [EPR6793] to ATP7b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human ATP7b aa 150-250.

  • Positive control

    • WB: Caco-2 lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 157 kDa.

Target

  • Function

    Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
  • Tissue specificity

    Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
  • Involvement in disease

    Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
    Contains 6 HMA domains.
  • Post-translational
    modifications

    Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
  • Cellular localization

    Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP7B antibody
    • ATP7B_HUMAN antibody
    • ATPase, Cu(2+) transporting, beta polypeptide antibody
    • ATPase, Cu++ transporting, beta polypeptide antibody
    • Copper pump 2 antibody
    • Copper transporting ATPase 2 antibody
    • PWD antibody
    • Toxic milk antibody
    • tx antibody
    • WC1 antibody
    • WD antibody
    • Wilson disease associated protein antibody
    • Wilson disease-associated protein antibody
    • WND antibody
    • WND/140 kDa antibody
    see all

Images

  • Anti-ATP7b antibody [EPR6793] (ab131208) at 1/1000 dilution + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 157 kDa
    Observed band size: 165 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID: 26438057 and 18673401 

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

ab131208 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Human fibroblasts)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Human fibroblasts
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 21 2018

Question
Answer

Thanks for your call today and for your enquiry.

Since our ATP7b antibodies have not been tested for cross-reactivity with rat, by participating in our AbTrial program you can try one of these antibodies in rat without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know which ATP7b antibody you're interested in testing.

2. I'll email you an inactive personal discount code for the value of the product.

3. Purchase and test the antibody at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

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