Overview

  • Product name

    Anti-ATP7b antibody [EPR6794]
    See all ATP7b primary antibodies
  • Description

    Rabbit monoclonal [EPR6794] to ATP7b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATP7b aa 1400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P35670

  • Positive control

    • WB: Caco-2 and HepG2 whole cell lysate (ab7900). FC: HepG2 cells. ICC/IF: HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 157 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
    • Tissue specificity

      Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
    • Involvement in disease

      Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis.
    • Sequence similarities

      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
      Contains 6 HMA domains.
    • Post-translational
      modifications

      Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
    • Cellular localization

      Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP7B antibody
      • ATP7B_HUMAN antibody
      • ATPase, Cu(2+) transporting, beta polypeptide antibody
      • ATPase, Cu++ transporting, beta polypeptide antibody
      • Copper pump 2 antibody
      • Copper transporting ATPase 2 antibody
      • PWD antibody
      • Toxic milk antibody
      • tx antibody
      • WC1 antibody
      • WD antibody
      • Wilson disease associated protein antibody
      • Wilson disease-associated protein antibody
      • WND antibody
      • WND/140 kDa antibody
      see all

    Images

    • Anti-ATP7b antibody [EPR6794] (ab124973) at 1/10000 dilution (Purified) + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 157 kDa
      Observed band size: 157 kDa

    • Anti-ATP7b antibody [EPR6794] (ab124973) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 157 kDa
      Observed band size: 157 kDa

    • Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATP7b with purified ab124973 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATP7b with purified ab124973 at 1:100 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 �g/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 �g/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow cytometric analysis of permeabilized HepG2 cells using unpurified anti-ATP7B RabMAb in red (ab124973) or a rabbit IgG in green(negative).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Li J  et al. miR-186 reverses cisplatin resistance and inhibits the formation of the glioblastoma-initiating cell phenotype by degrading Yin Yang 1 in glioblastoma. Int J Mol Med 43:517-524 (2019). Read more (PubMed: 30365062) »
    • Kakuda M  et al. Copper ions are novel therapeutic agents for uterine leiomyosarcoma. Am J Obstet Gynecol N/A:N/A (2019). Read more (PubMed: 31351063) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human iPS cells differentiated into neurons)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    human iPS cells differentiated into neurons
    Blocking step
    Odyssey blocking solution from Li-Cor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Jan 15 2018

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