Recombinant
RabMAb

Recombinant Anti-ATP7b antibody [EPR6794] - BSA and Azide free (ab240011)

Overview

  • Product name

    Anti-ATP7b antibody [EPR6794] - BSA and Azide free
    See all ATP7b primary antibodies
  • Description

    Rabbit monoclonal [EPR6794] to ATP7b - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATP7b aa 1450-1550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Caco-2 and HepG2 whole cell lysate (ab7900). FC: HepG2 cells. ICC/IF: HepG2 cells.
  • General notes

    ab240011 is the carrier-free version of ab124973 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240011 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 157 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
    • Tissue specificity

      Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
    • Involvement in disease

      Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis.
    • Sequence similarities

      Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
      Contains 6 HMA domains.
    • Post-translational
      modifications

      Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
    • Cellular localization

      Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP7B antibody
      • ATP7B_HUMAN antibody
      • ATPase, Cu(2+) transporting, beta polypeptide antibody
      • ATPase, Cu++ transporting, beta polypeptide antibody
      • Copper pump 2 antibody
      • Copper transporting ATPase 2 antibody
      • PWD antibody
      • Toxic milk antibody
      • tx antibody
      • WC1 antibody
      • WD antibody
      • Wilson disease associated protein antibody
      • Wilson disease-associated protein antibody
      • WND antibody
      • WND/140 kDa antibody
      see all

    Images

    • Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATP7b with purified ab124973 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide�(ab124973)
    • Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ATP7b with purified ab124973 at 1:100 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 �g/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 �g/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide�(ab124973)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124973).

    • Flow cytometric analysis of permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells using unpurified anti-ATP7B RabMAb in red (ab124973) or a rabbit IgG in green(negative).

      This data was developed using the same antibody clone in a different buffer formulation containing glycerol, tris glycine, tissue culture supernatant and sodium azide (ab124973).

    References

    ab240011 has not yet been referenced specifically in any publications.

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