Recombinant
RabMAb

Recombinant Anti-ATP7b antibody [EPR6794] (Phycoerythrin) (ab211985)

Overview

  • Product name

    Anti-ATP7b antibody [EPR6794] (Phycoerythrin)
    See all ATP7b primary antibodies
  • Description

    Rabbit monoclonal [EPR6794] to ATP7b (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ATP7b aa 1450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P35670

  • Positive control

    • Flow Cytometry: HepG2 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
  • Tissue specificity

    Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
  • Involvement in disease

    Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis.
  • Sequence similarities

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
    Contains 6 HMA domains.
  • Post-translational
    modifications

    Isoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form.
  • Cellular localization

    Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP7B antibody
    • ATP7B_HUMAN antibody
    • ATPase, Cu(2+) transporting, beta polypeptide antibody
    • ATPase, Cu++ transporting, beta polypeptide antibody
    • Copper pump 2 antibody
    • Copper transporting ATPase 2 antibody
    • PWD antibody
    • Toxic milk antibody
    • tx antibody
    • WC1 antibody
    • WD antibody
    • Wilson disease associated protein antibody
    • Wilson disease-associated protein antibody
    • WND antibody
    • WND/140 kDa antibody
    see all

Images

  • Overlay histogram showing HepG2 cells stained with ab211985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211985, 1/1000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter. 

    This antibody gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10min), permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab211985 has not yet been referenced specifically in any publications.

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