Anti-ATP8A1 antibody (ab234760)
Key features and details
- Rabbit polyclonal to ATP8A1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP8A1 antibody -
Description
Rabbit polyclonal to ATP8A1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human ATP8A1 aa 400-550.
Database link: Q9Y2Q0 -
Positive control
- IHC-P: Human cervical cancer and appendix tissues. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
May play a role in the transport of aminophospholipids from the outer to the inner leaflet of various membranes and the maintenance of asymmetric distribution of phospholipids, mainly in secretory vesicles. -
Tissue specificity
Found in most adult tissues except liver, testis and placenta. Most abundant in heart, brain and skeletal muscle. Also detected in fetal tissues. Isoform 1 is only detected in brain, skeletal muscle and heart and is the most abundant form in skeletal muscle. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. - Information by UniProt
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Database links
- Entrez Gene: 317692 Cow
- Entrez Gene: 10396 Human
- Entrez Gene: 11980 Mouse
- Omim: 609542 Human
- SwissProt: Q29449 Cow
- SwissProt: Q9Y2Q0 Human
- SwissProt: P70704 Mouse
- Unigene: 435052 Human
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Alternative names
- AT8A1_HUMAN antibody
- Atp8a1 antibody
- ATPase class I type 8A member 1 antibody
see all
Images
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Paraffin-embedded human appendix tissue stained for ATP8A1 using ab234760 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human cervical cancer tissue stained for ATP8A1 using ab234760 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ATP8A1 (green) using ab234760 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488 conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234760 has not yet been referenced specifically in any publications.