Anti-ATP8B1 antibody (ab121576)
Key features and details
- Rabbit polyclonal to ATP8B1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP8B1 antibody
See all ATP8B1 primary antibodies -
Description
Rabbit polyclonal to ATP8B1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
KIWVLTGDKK ETAENIGFAC ELLTEDTTIC YGEDINSLLH ARMENQRNRG GVYAKFAPPV QESFFPPGGN RALII internal sequence amino acids 727-801 of Human ATP8B1.
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Positive control
- IHC-P: Human lymph node, liver, kidney and cerebral cortex tissue.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
Target
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Function
May play a role in the transport of aminophospholipids from the outer to the inner leaflet of various membranes and the maintenance of asymmetric distribution of phospholipids in the canicular membrane. May have a role in transport of bile acids into the canaliculus, uptake of bile acids from intestinal contents into intestinal mucosa or both. -
Tissue specificity
Found in most tissues except brain and skeletal muscle. Most abundant in pancreas and small intestine. -
Involvement in disease
Defects in ATP8B1 are the cause of progressive familial intrahepatic cholestasis type 1 (PFIC1) [MIM:211600]; also known as Byler disease. PFIC1 is an autosomal recessive disorder, characterized by early infancy cholestasis, that may be initially episodic but progresses to malnutrition, growth retardation and end-stage liver disease before adulthood.
Defects in ATP8B1 are the cause of benign recurrent intrahepatic cholestasis type 1 (BRIC1) [MIM:243300]; also known as Summerskill syndrome. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration. Patients are asymptomatic between episodes, both clinically and biochemically.
Defects in ATP8B1 can be associated with intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as pregnancy-related cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5205 Human
- Omim: 602397 Human
- SwissProt: O43520 Human
- Unigene: 216623 Human
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Alternative names
- AT8B1_HUMAN antibody
- ATP8B1 antibody
- ATPase class I type 8B member 1 antibody
see all
Images
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Immunohistochemical analysis of human cerebral cortex tissue labeling ATP8B1 with ab121576 at 1/20 dilution.
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Immunohistochemical analysis of human kidney tissue labeling ATP8B1 with ab121576 at 1/20 dilution.
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Immunohistochemical analysis of human liver tissue labeling ATP8B1 with ab121576 at 1/20 dilution.
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Immunohistochemical analysis of human lymph node tissue labeling ATP8B1 with ab121576 at 1/20 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121576 has not yet been referenced specifically in any publications.