Overview

  • Product name

  • Description

    Rabbit polyclonal to ATP9A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human ATP9A aa 2-69.
    Sequence:

    TDNIPLQPVRQKKRMDSRPRAGCCEWLRCCGGGEARPRTVWLGHPEKRDQ RYPRNVINNQKYNFFTFL


    Database link: O75110

  • Positive control

    • WB: MCF7 and HepG2 cell lysate. IHC-P: Human brain and testis tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 119 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

Images

  • All lanes : Anti-ATP9A antibody (ab234873) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 119 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATP9A (green) using ab234873 at 1/100 dilution in ICC/IF. Secondary antibody was an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).

  • Paraffin-embedded human testis tissue stained for ATP9A using ab234873 at 1/100 dilution in immunohistochemical anaysis.

  • Paraffin-embedded human brain tissue stained for ATP9A using ab234873 at 1/100 dilution in immunohistochemical anaysis.

References

ab234873 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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