Product nameATPase Assay Kit
Assay typeEnzyme activity (quantitative)
Assay durationMultiple steps standard assay
ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLockTM gives a stable end-point signal and is not prone to precipitation. PiColorLockTM has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis.
This product is manufactured by Expedeon, an Abcam company. It was previously called ATPase Enzyme Assay Kit. Expedeon product code 601-0120 is the same as the 2 x 96 tests size of this product.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsPlease refer to protocols.
Components 2 x 96 tests 0.1 M Magnesium chloride 1 x 1.5ml 0.1 mM Pi Standard 1 x 5ml 0.5 M Tris pH 7.5 1 x 5ml 96-well plates 2 units Accelerator 1 x 0.25ml ATP (lyophilized) 4 vials PiColorLock 1 x 10ml Stabilizer 1 x 5ml
Our Abpromise guarantee covers the use of ab270551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Data provided for demonstration purposes only.
Standard Curve for ATPase Assay Kit (ab270551).
ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ provides baseline readings.
Dynamic Range for ATPase Assay Kit (ab270551).
PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.
ab270551 has not yet been referenced specifically in any publications.