Key features and details
- Rabbit polyclonal to ATPase Inhibitory Factor 1/IF1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATPase Inhibitory Factor 1/IF1 antibody
See all ATPase Inhibitory Factor 1/IF1 primary antibodies
DescriptionRabbit polyclonal to ATPase Inhibitory Factor 1/IF1
Specificityab197806 detects endogenous levels of total ATPase Inhibitory Factor 1 / IF1 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Fusion protein corresponding to Human ATPase Inhibitory Factor 1/IF1 aa 26-106 (internal sequence).
Database link: Q9UII2
- HeLa, Jurkat, MCF7 and A431 cell lyates; Human thyroid cancer tissue.
Previously labelled as ATPase Inhibitory Factor 1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 12 kDa.|
FunctionEndogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
Sequence similaritiesBelongs to the ATPase inhibitor family.
modificationsExhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
- Information by UniProt
- ATIF1_HUMAN antibody
- ATP synthase inhibitor protein antibody
- ATPase inhibitor antibody
All lanes : Anti-ATPase Inhibitory Factor 1/IF1 antibody (ab197806) at 1/200 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : MCF7 cell lysates
Lane 4 : A431 cell lysates
Lysates/proteins at 40 µg per lane.
Predicted band size: 12 kDa
Exposure time: 2 minutes
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling ATPase Inhibitory Factor 1/IF1 with ab197806 at a 1/50 dilution.
ab197806 has not yet been referenced specifically in any publications.