Key features and details
- Rabbit polyclonal to ATPase Inhibitory Factor 1/IF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATPase Inhibitory Factor 1/IF1 antibody
See all ATPase Inhibitory Factor 1/IF1 primary antibodies
DescriptionRabbit polyclonal to ATPase Inhibitory Factor 1/IF1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: Human heart tissue IHC-P: Human duodenum, rectum, kidney, testis, and fallopian tube tissue. ICC/IF: U-2 OS cells.
Previously labelled as ATPase Inhibitory Factor 1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 12 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionEndogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
Sequence similaritiesBelongs to the ATPase inhibitor family.
modificationsExhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
- Information by UniProt
- ATIF1_HUMAN antibody
- ATP synthase inhibitor protein antibody
- ATPase inhibitor antibody
Anti-ATPase Inhibitory Factor 1/IF1 antibody (ab223779) at 0.4 µg/ml + Human heart tissue
Predicted band size: 12 kDa
Paraffin-embedded human rectum tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ATPase Inhibitory Factor 1/IF1 (green) using ab223779 at 4 µg/ml in ICC/IF.
Paraffin-embedded human kidney tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human fallopian tube tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human duodenum tissue stained for ATPase Inhibitory Factor 1/IF1 using ab223779 at 1/50 dilution in immunohistochemical analysis.
ab223779 has not yet been referenced specifically in any publications.