Key features and details
- Rabbit polyclonal to ATPase Inhibitory Factor 1/IF1
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ATPase Inhibitory Factor 1/IF1 antibody
See all ATPase Inhibitory Factor 1/IF1 primary antibodies
DescriptionRabbit polyclonal to ATPase Inhibitory Factor 1/IF1
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
Previously labelled as ATPase Inhibitory Factor 1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab81061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.|
FunctionEndogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
Sequence similaritiesBelongs to the ATPase inhibitor family.
modificationsExhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
- Information by UniProt
- ATIF1_HUMAN antibody
- ATP synthase inhibitor protein antibody
- ATPase inhibitor antibody
Anti-ATPase Inhibitory Factor 1/IF1 antibody (ab81061) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab81061 has been referenced in 1 publication.
- Capparelli C et al. CTGF drives autophagy, glycolysis and senescence in cancer-associated fibroblasts via HIF1 activation, metabolically promoting tumor growth. Cell Cycle 11:2272-84 (2012). ICC/IF ; Human . PubMed: 22684333