Product nameATPase/GTPase Assay Kit
ATPase/GTPase Assay Kit (ab272520) offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 - 660 nm).
High sensitivity: detection of 0.007 U/L ATPase or GTPase activity.
Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes.
Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 200 tests Assay Buffer 1 x 10ml Pi Standard (1 mM) 1 x 1ml Reagent 1 x 50ml
Our Abpromise guarantee covers the use of ab272520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Phosphate assays in 96-well format.
1. H2O, 2. Phosphate, 3. ATP in H2O, 4. ATP/Phosphate in H2O, 5, 7, 9, 11, 13: ATP in Assay Buffer with, where indicated, 0.5 mg/mL BSA, 1 mM DTT, 2 mM β-mercaptoethanol (βME) and 5% DMSO. 6, 8, 10, 12, 14: ATP/Phosphate in Assay Buffer. Phosphate and ATP were at 50 µM and 1 mM, respectively. The assay is not affected by these component.
ab272520 has not yet been referenced specifically in any publications.